首页> 美国卫生研究院文献>The Journal of Biological Chemistry >A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain (CTD)

【2h】

A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain (CTD)

机译：转录延伸因子Spt6中的串联SH2域绑定了磷酸化的RNA聚合酶II C末端重复域（CTD）

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

Spt6 is an essential transcription elongation factor and histone chaperone that binds the C-terminal repeat domain (CTD) of RNA polymerase II. We show here that Spt6 contains a tandem SH2 domain with a novel structure and CTD-binding mode. The tandem SH2 domain binds to a serine 2-phosphorylated CTD peptide in vitro, whereas its N-terminal SH2 subdomain, which we previously characterized, does not. CTD binding requires a positively charged crevice in the C-terminal SH2 subdomain, which lacks the canonical phospho-binding pocket of SH2 domains and had previously escaped detection. The tandem SH2 domain is apparently required for transcription elongation in vivo as its deletion in cells is lethal in the presence of 6-azauracil.

机译：Spt6是必不可少的转录延伸因子和组蛋白伴侣，与RNA聚合酶II的C末端重复域（CTD）结合。我们在这里显示，Spt6包含具有新颖结构和CTD结合模式的串联SH2域。串联SH2结构域在体外与丝氨酸2磷酸化的CTD肽结合，而我们先前表征的N末端SH2子结构域则没有。 CTD绑定需要C端SH2子域中带正电荷的缝隙，该缝隙缺少SH2域的规范性磷酸结合口袋，并且以前无法进行检测。串联SH2结构域显然是体内转录延伸所必需的，因为在6-氮杂嘧啶存在下，其在细胞中的缺失是致命的。

著录项

期刊名称 The Journal of Biological Chemistry
作者
Mai Sun; Laurent Larivière; Stefan Dengl; Andreas Mayer; Patrick Cramer;
展开▼
作者单位

展开▼
年(卷),期 2010(285),53
年度 2010
页码 41597–41603
总页数 7
原文格式 PDF
正文语种
中图分类分子生物学;
关键词
Chromatin Crystal Structure Peptide Interactions RNA Polymerase II Transcription Elongation Factors;

机译：染色质;晶体结构;肽相互作用;RNA聚合酶II;转录延伸因子;

相似文献

外文文献
中文文献
专利

1. A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain (CTD) [J] . Mai Sun, Laurent Larivière, Stefan Dengl, The Journal of biological chemistry . 2010,第53期

机译：转录伸长因子SPT6中的串联SH2结构域结合磷酸化的RNA聚合酶II C-末端重复域（CTD）
2. Solution Structure of Tandem SH2 Domains from Spt6 Protein and Their Binding to the Phosphorylated RNA Polymerase II C-terminal Domain [J] . Jianping Liu, Jiahai Zhang, Qingguo Gong, The Journal of biological chemistry . 2011,第33期

机译：SPT6蛋白的串联SH2结构域的溶液结构及其与磷酸化RNA聚合酶II C-末端结构域的结合
3. Separable Functions of the Fission Yeast Spt5 Carboxyl-Terminal Domain (CTD) in Capping Enzyme Binding and Transcription Elongation Overlap with Those of the RNA Polymerase II CTD [J] . Susanne Schneider, Yi Pei, Stewart Shuman, Molecular and Cellular Biology . 2010,第10期

机译：裂变酵母Spt5羧基末端域（CTD）与RNA聚合酶II CTD在上限酶结合和转录延伸重叠中的独立功能
4. The CrkRS/CDK12 kinase is activated by a novel isoform of Cyclin K and phosphorylates and the C-terminal domain of RNA Pol II [C] . Annie Moradian, S.-W. Grace Cheng, Michael Kuzyk, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：CrKRS / CDK12激酶通过细胞周期蛋白K和磷酸化合物的新型同种型和RNA POL II的C末端结构域激活
5. Characterization of the Association of mRNA Export Factor Yra1 with the C-terminal Domain of RNA Polymerase II in vivo and in vitro [D] . MacKellar, April Lee 2011

机译：mRNA出口因子Yra1与RNA聚合酶II的C末端域在体内和体外的关联的表征。
6. Solution Structure of Tandem SH2 Domains from Spt6 Protein and Their Binding to the Phosphorylated RNA Polymerase II C-terminal Domain [O] . Jianping Liu, Jiahai Zhang, Qingguo Gong, 2011

机译：Spt6蛋白的串联SH2域的溶液结构及其与磷酸化RNA聚合酶II C末端域的结合
7. A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain (CTD)* [O] . Sun, Mai, Larivière, Laurent, Dengl, Stefan, 2010

机译：转录延伸因子Spt6中的串联SH2结构域与磷酸化的RNA聚合酶II C末端重复结构域（CTD）绑定*

A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain (CTD)

摘要

著录项

相似文献

相关主题

期刊订阅