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首页> 美国卫生研究院文献>Animal Nutrition >Low-protein diets supplemented with glutamic acid or aspartic acid ameliorate intestinal damage in weaned piglets challenged with hydrogen peroxide
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Low-protein diets supplemented with glutamic acid or aspartic acid ameliorate intestinal damage in weaned piglets challenged with hydrogen peroxide

机译：补充有谷氨酸或天冬氨酸的低蛋白质饮食在断奶仔猪遭到过氧化氢的断奶仔猪中改善肠道损伤

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Glutamic acid (Glu) and aspartic acid (Asp) are acidic amino acids with regulatory roles in nutrition, energy metabolism, and oxidative stress. This study aimed to evaluate the effects of low-protein diets supplemented with Glu and Asp on the intestinal barrier function and energy metabolism in weaned piglets challenged with hydrogen peroxide (H2O2). Forty piglets were randomly divided into 5 groups: NC, PC, PGA, PG, and PA (n = 8 for each group). Pigs in the NC and PC groups were fed a low-protein diet, while pigs in the PGA, PG, or PA groups were fed the low-protein diet supplemented with 2.0% Glu +1.0% Asp, 2.0% Glu, or 1.0% Asp, respectively. On day 8 and 11, pigs in the NC group were intraperitoneally injected with saline (1 mL/kg BW), while pigs in the other groups were intraperitoneally administered 10% H2O2 (1 mL/kg BW). On day 14, all pigs were sacrificed to collect jejunum and ileum following the blood sample collection in the morning. Notably, low-protein diets supplemented with Glu or Asp ameliorated the intestinal oxidative stress response in H2O2-challenged piglets by decreasing intestinal expression of genes (P < 0.05) (e.g., manganese superoxide dismutase [MnSOD], glutathione peroxidase [Gpx]-1, and Gpx-4) encoding oxidative stress-associated proteins, reducing the serum concentration of diamine oxidase (P < 0.05), and inhibiting apoptosis of the intestinal epithelium. Glu and Asp supplementation attenuated the upregulated expression of energy metabolism-associated genes (such as hexokinase and carnitine palmitoyltransferase-1) and the H2O2-induced activation of acetyl-coenzyme A carboxylase (ACC) in the jejunum and adenosine monophosphate-activated protein kinase–acetyl-ACC signaling in the ileum. Dietary Glu and Asp also ameliorated intestinal barrier damage as indicated by restored intestinal histology and morphology. In conclusion, low-protein diets supplemented with Glu and Asp protected against oxidative stress-induced intestinal dysfunction in piglets, suggesting that this approach could be used as a nutritional regulatory protectant against oxidative stress.
机译：谷氨酸（Glu）和天冬氨酸（ASP）是酸性氨基酸，具有营养，能量代谢和氧化应激的调节作用。本研究旨在评估含有Glu和Asp的低蛋白饮食对用过氧化氢（H2O2）攻击的断奶仔猪的肠道阻隔功能和能量代谢的影响。将四十只仔猪随机分为5组：NC，PC，PGA，PG和PA（每组n = 8）。 NC和PC组中的猪被喂养低蛋白质饮食，而PGA，PG或PA组的猪喂入补充2.0％Glu + 1.0％ASP，2.0％Glu或1.0％的低蛋白质饮食分别为ASP。在第8和11天，NC组中的猪腹腔内注射盐水（1ml / kg bw），而其他基团的猪腹膜内施用10％H 2 O 2（1ml / kg Bw）。在第14天，牺牲所有猪在早晨血液样本收集后收集Jejunum和回肠。值得注意的是，通过降低基因的肠道表达（例如，锰超氧化物歧化酶[mnsod]，谷胱甘肽过氧化物酶[GPX] -1，补充有Glu或Asp的低蛋白质饮食改善了H2O2攻击仔猪中的肠道氧化应激反应编码氧化应激相关蛋白的GPX-4），降低二胺氧化酶的血清浓度（P <0.05），抑制肠上皮的凋亡。 Glu和Asp补充衰减了能量代谢相关基因的上调表达（例如六酮酶和肉毒氨基棕榈酰转移酶-1），以及在JEJUNUM和腺苷的甲基酶A羧基活化蛋白激酶 - 激活蛋白激酶 - 中的H2O2诱导的乙酰 - 辅酶A羧基（ACC）的活化。乙酰-ACC信号在回肠中。如恢复的肠道组织学和形态学所示，膳食Glu和Asp也改善了肠道阻挡损伤。总之，补充有Glu和ASP的低蛋白质饮食免受仔猪氧化应激诱导的肠功能障碍，表明这种方法可以用作氧化胁迫的营养调节保护剂。

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