• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >High-throughput optimization of the chemically defined synthetic medium for the production of erythromycin A
【24h】

High-throughput optimization of the chemically defined synthetic medium for the production of erythromycin A

机译：用于生产红霉素A的化学成分确定的合成培养基的高通量优化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Erythromycin A is an important antibiotic. A chemically defined synthetic medium for erythromycin production was systematically optimized in this study. A high-throughput method was employed to reduce the number of components and optimize the concentration of each component. After two round single composition deletion experiment, only 19 components were remained in the medium, and then the concentration of each component was optimized through PB experiment. The optimal medium from the PB experiment was further optimized according to the nitrogen and phosphate metabolic consumption in 5 L bioreactor. It was observed that among the 8 amino acids concluded in the media, 4 amino acids were first consumed, when they are almost depleted, the other 4 amino acids were initiated their consumption afterwards in 5 L bioreactor. The decrease of phosphate concentration would increase q(glc) and q(ery). However, when phosphate concentration was too low, the production of erythromycin was hindered. The positive correlation between intracellular metabolite pools and Y-ery/glc indicated that low phosphate concentration in the medium can promote cell metabolism especially secondary metabolism during the stationary phase; however, if it was too low (5 mmol/L), the cell metabolism and secondary metabolism would both slow down. The erythromycin titer in the optimized medium (medium V) reached 1380 mg/L, which was 17 times higher than the previously used synthetic medium in our lab. The optimized medium can facilitate the metabolomics study or metabolic flux analysis of the erythromycin fermentation process, which laid a solid foundation for further study of erythromycin fermentation process.
机译：红霉素A是重要的抗生素。在这项研究中，系统地优化了用于生产红霉素的化学定义的合成培养基。采用高通量方法来减少组分的数量并优化每种组分的浓度。经过两轮单一成分缺失实验，培养基中仅残留19种成分，然后通过PB实验优化各成分的浓度。根据5 L生物反应器中氮和磷的代谢消耗，进一步优化了PB实验的最佳培养基。观察到，在培养基中总结的8种氨基酸中，首先消耗了4种氨基酸，当它们几乎被耗尽时，其余4种氨基酸随后在5 L生物反应器中开始消耗。磷酸盐浓度的降低会增加q（glc）和q（ery）。然而，当磷酸盐浓度太低时，红霉素的产生受到阻碍。细胞内代谢物库与Y-ery / glc之间呈正相关，表明培养基中低浓度的磷酸盐可以促进细胞代谢，特别是在稳定期的次生代谢。但是，如果过低（5 mmol / L），细胞代谢和次级代谢都会减慢。优化培养基（中度V）中的红霉素滴度达到1380 mg / L，比我们实验室以前使用的合成培养基高17倍。优化的培养基可以促进红霉素发酵过程的代谢组学研究或代谢通量分析，为进一步研究红霉素发酵过程奠定了坚实的基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号