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Black lipid membranes stabilized through substrate conjugation to a hydrogel

机译：黑色脂质膜可通过底物与水凝胶结合而稳定

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Recent research in stabilizing lipid bilayer membranes has been directed toward tethering the membrane to a solid surface or contacting the membrane with a solid support such as a gel. It is also known that the solvent annulus plays an important role in lipid bilayer stability. In this work, the authors set out to stabilize the solvent annulus. Glass substrates with ～500 μm apertures were functionalized with 3-methacryloxypropyltrimethoxysilane to allow cross-linking with a surrounding polyethyleneglycol dimethacrylate hydrogel. The hydrogel makes a conformal mold around both the lipid bilayer and the solvent reservoir. Since the hydrogel is covalently conjugated with the glass substrate via vinyl groups, the solvent annulus is prevented from leaving the aperture boundary. Measurements of a membrane created with this approach showed that it remained a stable bilayer with a resistance greater than 1 GΩ( for 12 days. Measurements of the ion channel gramicidin A, α-hemolysin, and alamethicin incorporated into these membranes showed the same conductance behavior as conventional membranes.
机译：稳定脂质双层膜的最新研究已针对将膜束缚到固体表面或使膜与固体支持物如凝胶接触。还已知溶剂环在脂质双层稳定性中起重要作用。在这项工作中，作者着手稳定溶剂环。用3-甲基丙烯酰氧基丙基三甲氧基硅烷对孔径约为500μm的玻璃基板进行功能化，以使其与周围的聚乙二醇二甲基丙烯酸酯水凝胶发生交联。水凝胶在脂质双层和溶剂储器周围形成保形模具。由于水凝胶经由乙烯基与玻璃基板共价结合，因此防止了溶剂环离开孔边界。通过这种方法制成的膜的测量结果表明，该膜保持了稳定的双层，电阻大于1GΩ（持续12天），并测量了掺入这些膜中的离子通道短杆菌肽A，α-溶血素和缩水甘油的电导率作为常规膜。

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