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首页> 外文期刊>BMC Biotechnology >Thrombolytic effects of Douchi Fibrinolytic enzyme from Bacillus subtilis LD-8547 in vitro and in vivo
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Thrombolytic effects of Douchi Fibrinolytic enzyme from Bacillus subtilis LD-8547 in vitro and in vivo

机译：枯草芽孢杆菌LD-8547的豆chi纤溶酶的体内外溶栓作用

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Background Today, thrombosis is one of the most widely occurring diseases in modern life. Drugs with thrombolytic functions are the most effective methods in the treatment of thrombosis. Among them, Douchi fibrinolytic enzyme (DFE) is a promising agent. DFE was isolated from Douchi , a typical and popular soybean-fermented food in China, and it can dissolve fibrin directly and efficiently. A strain, Bacillus subtilis LD-8547 produced DFE with high fibrinolytic activity has been isolated in our lab previously. Results In the study, thrombolytic effect of DFE from Bacillus subtilis LD-8547 was studied in vitro and in vivo systematically. The results showed that DFE played a significant role in thrombolysis and anticoagulation in vitro. And the thrombolytic effects correlated with DFE in a dose-dependent manner. In vivo, the acute toxicity assay showed that DFE had no obvious acute toxicity to mice. Test of carrageenan-induced thrombosis in mice indicated that the DFE significantly prevented tail thrombosis, and arterial thrombosis model test indicated that Douchi fibrinolytic enzyme DFE had thrombolytic effect on carotid thrombosis of rabbits in vivo. Other results in vivo indicated that DFE could increase bleeding and clotting time obviously. Conclusions The DFE isolated from Bacillus subtilis LD-8547 has obvious thrombolytic effects in vitro and in vivo. This function demonstrates that this enzyme can be a useful tool for preventing and treating clinical thrombus.
机译：背景技术如今，血栓形成是现代生活中最广泛发生的疾病之一。具有溶栓功能的药物是治疗血栓形成的最有效方法。其中，豆chi纤溶酶（DFE）是一种有前途的药物。 DFE是从豆Dou中分离出来的，豆chi是中国典型的大豆发酵食品，它可以直接有效地溶解纤维蛋白。以前在我们实验室中已经分离出了具有高纤溶活性的枯草芽孢杆菌LD-8547菌株。结果本研究对枯草芽孢杆菌LD-8547的DFE的溶栓作用进行了系统的体内外研究。结果表明，DFE在体外溶栓和抗凝中起着重要作用。并且溶栓作用与DFE呈剂量依赖关系。在体内，急性毒性试验表明DFE对小鼠没有明显的急性毒性。角叉菜胶诱发的小鼠血栓形成试验表明DFE显着预防了尾部血栓形成，动脉血栓形成模型试验表明Douchi纤溶酶DFE对家兔的颈动脉血栓形成具有溶栓作用。体内的其他结果表明，DFE可以明显增加出血和凝血时间。结论从枯草芽孢杆菌LD-8547中分离得到的DFE在体内外具有明显的溶栓作用。该功能证明该酶可以作为预防和治疗临床血栓的有用工具。

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