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首页> 外文期刊>BMC Microbiology >Small-scale analysis of exopolysaccharides from Streptococcus thermophilus grown in a semi-defined medium
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Small-scale analysis of exopolysaccharides from Streptococcus thermophilus grown in a semi-defined medium

机译：在半确定培养基中生长的嗜热链球菌胞外多糖的小规模分析

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摘要

Exopolysaccharides (EPSs) produced by lactic acid bacteria are important for the texture of fermented foods and have received a great deal of interest recently. However, the low production levels of EPSs in combination with the complex media used for growth of the bacteria have caused problems in the accurate analysis of the EPS. The purpose of this study was to find a growth medium for physiological studies of the lactic acid bacterium Streptococcus thermophilus, and to develop a simple method for qualitative and quantitative analysis of EPSs produced in this medium. A semi-defined polysaccharide medium was developed and evaluated on six strains of Streptococcus thermophilus. The EPSs were analysed using a novel protocol incorporating ultracentrifugation for the removal of interfering sugars, hydrolysis and analysis of the monomer composition by High Performance Anion-Exchange Chromatography with pulsed amperometric detection. The medium and analysis method allowed accurate quantification and monomer analysis of 0.5 ml samples of EPSs from tube cultures. The presented medium should be useful for physiological studies of S. thermophilus, and, in combination with the method of analysis of EPS, will allow downscaling of physiological studies and screening for EPSs.
机译：乳酸菌产生的胞外多糖（EPS）对于发酵食品的质地很重要，并且最近引起了人们的极大兴趣。然而，EPS的低生产水平与用于细菌生长的复杂培养基的结合在EPS的准确分析中引起了问题。这项研究的目的是找到一种用于乳酸菌嗜热链球菌的生理研究的生长培养基，并开发一种定性和定量分析这种培养基中产生的EPS的简单方法。开发了半定义的多糖培养基，并在六株嗜热链球菌菌株上进行了评估。使用新型方案分析EPS，该方案包括超速离心以去除干扰糖，水解以及通过高效阴离子交换色谱和脉冲安培检测法分析单体组成。培养基和分析方法可以对来自管培养物的0.5 ml EPS样品进行准确的定量和单体分析。所介绍的培养基应可用于嗜热链球菌的生理学研究，并与EPS分析方法结合使用，可降低生理研究的规模并筛选EPS。

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