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首页> 外文期刊>British Biotechnology Journal >Molecular Differentiation of Five Quinoa (Chenopodium quinoa Willd.) Genotypes Using Inter-simple Sequence Repeat (ISSR) Markers
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Molecular Differentiation of Five Quinoa (Chenopodium quinoa Willd.) Genotypes Using Inter-simple Sequence Repeat (ISSR) Markers

机译：使用内部简单重复序列（ISSR）标记的五种藜麦（Chenopodium quinoa Willd。）基因型的分子分化

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Knowledge of genetic diversity is one of the important tools used for genetic management of quinoa accessions for plant breeding. This research aimed to molecularly characterize five quinoa genotypes using ISSR markers to reveal genetic polymorphism and identify unique markers for each genotype. Analysis of inter-simple sequence repeats (ISSR) revealed that 10 ISSR primers produced 53 amplicons, out of them 33 were polymorphic and the average percentage of polymorphism was 61.83%. The number of amplicons per primer ranged from 3 (HB-13, HB-10, HB-8 and 17898A) to 10 (HB-15) with an average of 5.3 fragments/primer across the different quinoa genotypes. Data showed a total number of unique ISSR markers of 24; eleven of them were positive and 13 were negative. Using ISSR analysis, we were able to identify some unique bands associated with quinoa genotypes. The genetic similarity ranged from 49% (between Ollague and each of QL-3 and Chipaya) to 76% (between CICA-17 and CO-407). The results indicated that all the five quinoa genotypes differ from each other at the DNA level where the average of genetic similarity (GS) between them was about 59%. The dendrogram separated the quinoa genotypes into two clusters; the first cluster included two genotypes (QL-3 and Chipaya). The second cluster was divided into two groups; the first group included two genotypes (CICA-17 and CO-407) and the second group included only one genotype (Ollague).
机译：遗传多样性知识是用于藜麦植物遗传育种的重要工具之一。这项研究旨在使用ISSR标记物对5种藜麦基因型进行分子表征，以揭示遗传多态性并为每种基因型识别独特的标记物。对简单序列间重复（ISSR）的分析表明，有10个ISSR引物产生了53个扩增子，其中33个是多态性的，平均多态性百分比为61.83％。每个引物的扩增子数量范围从3个（HB-13，HB-10，HB-8和17898A）到10个（HB-15），在不同的藜麦基因型中平均有5.3个片段/引物。数据显示独特的ISSR标记总数为24;其中有11例是正面的，有13例是负面的。使用ISSR分析，我们能够鉴定出一些与藜麦基因型相关的独特条带。遗传相似性介于49％（在Ollague与QL-3和Chipaya之间）到76％（在CICA-17与CO-407之间）之间。结果表明，所有五个藜麦基因型在DNA水平上彼此不同，它们之间的遗传相似性（GS）平均值约为59％。树状图将藜麦的基因型分为两个簇。第一个簇包括两个基因型（QL-3和Chipaya）。第二个集群分为两组;第二个集群分为两个组。第一组包括两种基因型（CICA-17和CO-407），第二组仅包括一种基因型（Ollague）。

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