...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Neuroscience >Primary Neuronal Precursors in Adult Crayfish Brain: Replenishment from a Non-neuronal Source
【24h】

Primary Neuronal Precursors in Adult Crayfish Brain: Replenishment from a Non-neuronal Source

机译：成年小龙虾脑中的主要神经元前体：从非神经源补充。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Adult neurogenesis, the production and integration of new neurons into circuits in the brains of adult animals, is a common feature of a variety of organisms, ranging from insects and crustaceans to birds and mammals. In the mammalian brain the 1st-generation neuronal precursors, the astrocytic stem cells, reside in neurogenic niches and are reported to undergo self-renewing divisions, thereby providing a source of new neurons throughout an animal's life. In contrast, our work shows that the 1st-generation neuronal precursors in the crayfish ( Procambarus clarkii ) brain, which also have glial properties and lie in a neurogenic niche resembling that of vertebrates, undergo geometrically symmetrical divisions and both daughters appear to migrate away from the niche. However, in spite of this continuous efflux of cells, the number of neuronal precursors in the crayfish niche continues to expand as the animals grow and age. Based on these observations we have hypothesized that (1) the neuronal stem cells in the crayfish brain are not self-renewing, and (2) a source external to the neurogenic niche must provide cells that replenish the stem cell pool. Results In the present study, we tested the first hypothesis using sequential double nucleoside labeling to track the fate of 1st- and 2nd-generation neuronal precursors, as well as testing the size of the labeled stem cell pool following increasing incubation times in 5-bromo-2'-deoxyuridine (BrdU). Our results indicate that the 1st-generation precursor cells in the crayfish brain, which are functionally analogous to neural stem cells in vertebrates, are not a self-renewing population. In addition, these studies establish the cycle time of these cells. In vitro studies examining the second hypothesis show that Cell Tracker? Green-labeled cells extracted from the hemolymph, but not other tissues, are attracted to and incorporated into the neurogenic niche, a phenomenon that appears to involve serotonergic mechanisms. Conclusions These results challenge our current understanding of self-renewal capacity as a defining characteristic of all adult neuronal stem cells. In addition, we suggest that in crayfish, the hematopoietic system may be a source of cells that replenish the niche stem cell pool.
机译：背景技术成年神经发生是成年动物大脑中新神经元产生和整合到回路中的新神经元，是昆虫，甲壳类动物，鸟类和哺乳动物等各种生物体的共同特征。在哺乳动物的大脑中，第一代神经元前体星形细胞干细胞位于神经源性壁and中，据报道会经历自我更新分裂，从而为动物的整个生命提供了新的神经元来源。相反，我们的工作表明，小龙虾（Procambarus clarkii）脑中的第一代神经元前体也具有神经胶质特性，并且位于类似于脊椎动物的神经源性利基中，经历几何对称的分裂两个女儿似乎都从利基市场迁移出去。然而，尽管细胞持续不断地流出，但随着动物的成长和衰老，小龙虾生态位中神经元前体的数量仍在继续增长。基于这些观察，我们假设（1）小龙虾大脑中的神经元干细胞无法自我更新，并且（2）神经源性利基外部来源必须提供补充干细胞库的细胞。结果在本研究中，我们使用顺序双核苷标记法测试了第一个假设，以追踪第1代和第2代神经元前体的命运，并进行了测试在5-溴2'-脱氧尿苷（BrdU）中孵育时间增加后，标记干细胞库的大小。我们的结果表明，小龙虾大脑中的第一代前体细胞在功能上类似于脊椎动物的神经干细胞，并不是自我更新的种群。另外，这些研究确定了这些细胞的周期时间。检验第二种假设的体外研究表明，Cell Tracker？从血淋巴而不是其他组织中提取的绿色标记的细胞被吸引并整合到神经源性利基中，这种现象似乎涉及血清素能机制。结论这些结果挑战了我们目前对自我更新能力的理解，而自我更新能力是所有成年神经元干细胞的定义特征。此外，我们建议在小龙虾中，造血系统可能是补充利基干细胞池的细胞来源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号