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Proteomic analysis of whey from bovine colostrum and mature milk

机译:牛初乳和成熟牛奶中的乳清的蛋白质组学分析

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The aim of this study was to standardize a methodology to obtain two-dimensional (2D) maps of whey proteins from bovine colostrum and mature milk, using two-dimensional electrophoresis, in order to identify the minor proteins by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS). A total of 38 proteins were identified, 20 spots in the colostrum whey and 18 in the mature milk whey; 5 of them were identified for the first time.
机译:这项研究的目的是标准化一种方法,使用二维电泳从牛初乳和成熟牛奶中获得乳清蛋白的二维(2D)图,以便通过基质辅助激光解吸/电离来鉴定次要蛋白串联飞行时间质谱(MALDI TOF / TOF MS)。总共鉴定出38种蛋白质,初乳乳清20个斑点,成熟乳清18个斑点;其中5个是首次识别。

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