• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Serodiagnostic Potential of Culture Filtrate Antigens of Mycobacterium tuberculosis
【24h】

Serodiagnostic Potential of Culture Filtrate Antigens of Mycobacterium tuberculosis

机译：结核分枝杆菌培养滤液抗原的血清学诊断潜力。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Our studies of the humoral responses of tuberculosis (TB) patients have defined the repertoire of culture filtrate antigens ofMycobacterium tuberculosis that are recognized by antibodies from cavitary and noncavitary TB patients and demonstrated that the profile of antigens recognized changes with disease progression (K. Samanich et al., J. Infect. Dis. 178:1534–1538, 1998). We have identified several antigens with strong serodiagnostic potential. In the present study we have evaluated the reactivity of cohorts of human immunodeficiency virus (HIV)-negative, smear-positive; HIV-negative, smear-negative; and HIV-infected TB patients, with three of the candidate antigens, an 88-kDa protein, antigen (Ag) 85C, and MPT32, and compared the reactivity of the same patient cohort with the 38-kDa antigen and Ag 85A. We have also compared the reactivity of native Ag 85C and MPT32 with their recombinant counterparts. The evaluation of the reactivity was done by a modified enzyme-linked immunosorbent assay described earlier (S. Laal et al., Clin. Diag. Lab. Immunol. 4:49–56, 1997), in which all sera are preadsorbed againstEscherichia coli lysates to reduce the levels of cross-reactive antibodies. Our results demonstrate that (i) antigens identified on the basis of their reactivity with TB patients' sera provide high sensitivities for serodiagnosis, (ii) recombinant Ag 85C and MPT32, expressed in E. coli, show reduced reactivity with human TB sera, and (iii) of the panel of antigens tested, the 88-kDa protein is the most promising candidate for serodiagnosis of TB in HIV-infected individuals. Moreover, these results reaffirm that both the extent of the disease and the bacterial load may play a role in determining the antigen profile recognized by antibodies.
机译：我们对结核病（TB）患者的体液反应的研究确定了结核分枝杆菌（）的培养物滤液抗原库，这些抗原被空洞和非空洞TB患者的抗体识别，并证明了抗原的特征可以识别变化（K. Samanich et al。，J. Infect。Dis。178：1534-1538，1998）。我们已经鉴定了几种具有强血清学诊断潜力的抗原。在本研究中，我们评估了人类免疫缺陷病毒（HIV）阴性，涂片阳性的人群的反应性;艾滋病毒阴性，涂片阴性;艾滋病毒感染的结核病患者，使用三种候选抗原，即88 kDa蛋白，抗原（Ag）85C和MPT32，并比较了同一患者队列与38 kDa抗原和Ag 85A的反应性。我们还比较了天然Ag 85C和MPT32与它们的重组对应物的反应性。通过较早前描述的改良的酶联免疫吸附测定法（S. Laal等人，Clin。Diag。Lab。Immunol。4：49-56，1997）对反应性进行评估，其中所有血清均预先针对大肠埃希氏菌裂解液，以降低交叉反应抗体的水平。我们的结果表明（i）根据与结核病患者血清反应性鉴定的抗原为血清诊断提供了高敏感性，（ii）在 E中表达的重组Ag 85C和MPT32。大肠杆菌显示出与人结核病血清的反应性降低，并且（iii）在检测的一系列抗原中，88 kDa蛋白是HIV感染者对结核病进行血清学诊断的最有希望的候选者。此外，这些结果再次证实，疾病的程度和细菌载量都可能在确定抗体识别的抗原谱中起作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号