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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Immunization of BALB/c Mice with Killed Neospora caninum Tachyzoite Antigen Induces a Type 2 Immune Response and Exacerbates Encephalitis and Neurological Disease
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Immunization of BALB/c Mice with Killed Neospora caninum Tachyzoite Antigen Induces a Type 2 Immune Response and Exacerbates Encephalitis and Neurological Disease

机译：杀死新孢子虫速殖子抗原的BALB / c小鼠免疫诱导2型免疫反应并加剧脑炎和神经系统疾病

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BALB/c mice were immunized subcutaneously with solubleNeospora caninum tachyzoite antigen (NSO) entrapped in nonionic surfactant vesicles (NISVs) or administered with Freund's complete adjuvant (FCA). Following virulent parasite challenge, groups of mice immunized with NSO and either NISVs or FCA had clinical neurological disease and increased numbers of brain lesions compared to groups of mice inoculated with FCA, NISVs, or phosphate-buffered saline (PBS) alone. Increased numbers of brain lesions were statistically significant only between mice immunized with NISV-NSO and NISV- or PBS-treated mice. Following parasite challenge, brain inflammatory infiltrates in all experimental and control groups of mice were relatively similar and consisted of compact infiltrates of macrophages admixed with various numbers of lymphoid cells. Increased brain lesions in NSO-immunized mice were associated with increased antigen-specific interleukin 4 (IL-4) secretion and increased IL-4:gamma interferon secretion ratios from splenocytes in vitro and increased antigen-specific immunoglobulin G1 (IgG1):IgG2a ratios in vivo. Thus, immunization with whole killed N. caninum antigen and either liposoidal or Freund's adjuvant induced a type 2 immune response that was associated with worsened disease. The present studies emphasize the need to identify specific N. caninum antigens or other delivery systems that will elicit protective immune responses to neosporosis.
机译：用包裹在非离子表面活性剂囊泡（NISV）中的可溶性新孢子虫速殖子抗原（NSO）皮下免疫BALB / c小鼠，或用弗氏完全佐剂（FCA）进行免疫。与单独接种FCA，NISV或磷酸盐缓冲盐水（PBS）的小鼠相比，用强力寄生虫攻击后，用NSO和NISVs或FCA免疫的小鼠组具有临床神经系统疾病，并且脑损伤的数量增加。仅在用NISV-NSO免疫的小鼠与用NISV或PBS处理的小鼠之间，脑部损伤的增加在统计学上具有统计学意义。在进行寄生虫攻击后，所有实验组和对照组的小鼠脑部炎症浸润相对相似，由紧密混合的巨噬细胞浸润液与各种数量的淋巴样细胞组成。 NSO免疫小鼠脑损伤的增加与体外脾细胞抗原特异性白介素4（IL-4）分泌增加和IL-4：γ干扰素分泌比率增加以及抗原特异性免疫球蛋白G1（IgG1）：IgG2a比率增加有关体内。因此，用全部杀死的 N进行免疫。犬抗原和脂质体或弗氏佐剂诱导的2型免疫反应与疾病恶化有关。本研究强调需要确定特定的 N。犬抗原或其他递送系统会引发对新孢子虫病的保护性免疫反应。

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