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Infection Exclusion of the Rickettsial Pathogen Anaplasma marginale in the Tick Vector Dermacentor variabilis

机译:壁虱病媒变种Dermacentor variabilis的Path病病原体无性体感染的排除

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Anaplasma marginale is a tick-borne, rickettsial cattle pathogen that is endemic in several areas of the United States. Recent studies (J. de la Fuente, J. C. Garcia-Garcia, E. F. Blouin, J. T. Saliki, and K. M. Kocan, Clin. Diagn. Lab. Immunol. 9:658-668, 2002) demonstrated that infection of cultured tick cells and bovine erythrocytes with one genotype of A. marginale excluded infection with other genotypes, a phenomenon referred to as infection exclusion. The present study was undertaken to confirm the phenomenon of infection exclusion of A. marginale genotypes in a tick vector, Dermacentor variabilis. Only one genotype of A. marginale (Virginia isolate) was detected by PCR in ticks that fed first on a calf infected with a Virginia isolate and second on a calf infected with an Oklahoma isolate. These studies demonstrate that infection exclusion of A. marginale genotypes also occurs in naturally infected ticks, as well as in cattle and cultured tick cells, and results in establishment of only one genotype per tick.
机译:边缘无浆膜是由tick传播的立克次氏牛病原体,在美国的多个地区都很流行。最近的研究(J. de la Fuente,JC Garcia-Garcia,EF Blouin,JT Saliki和KM Kocan,Clin.Diagn.Lab.Immunol.9:658-668,2002)证明感染了培养的壁虱细胞和牛红细胞有一个 A基因型。边缘排除了其他基因型的感染,这种现象被称为感染排除。进行本研究以证实排除 A的现象。 a矢量 Dermacentor variabilis 中的边缘基因型。 A只有一种基因型。通过PCR在in中检测到(弗吉尼亚分离株),tick中首先喂食感染了弗吉尼亚分离株的小牛,其次喂食了感染俄克拉何马分离株的小牛。这些研究表明, A的感染被排除在外。边缘基因型也发生在自然感染的tick中,也存在于牛和培养的tick细胞中,导致每个tick只建立一种基因型。

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