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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Identification of B- and T-Cell Epitopes of BB, a Carrier Protein Derived from the G Protein of Streptococcus Strain G148
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Identification of B- and T-Cell Epitopes of BB, a Carrier Protein Derived from the G Protein of Streptococcus Strain G148

机译：BB的B和T细胞表位的鉴定，BB是链球菌菌株G148的G蛋白衍生的载体蛋白。

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Most conventional vaccines consist of killed organisms or purified antigenic proteins. Such molecules are generally poorly immunogenic and need to be coupled to carrier proteins. We have identified a new carrier molecule, BB, derived from the G protein of Streptococcus strain G148. We show that BB is able to induce strong antibody responses when conjugated to peptides or polysaccharides. In order to localize T and B cell epitopes in BB and match them with the albumin-binding region of the molecule, we immunized mice with BB, performed B and T pepscan analyses, and compared the results with pepscan done with sera and cells from humans. Our results indicate that BB has two distinct T helper epitopes, seven linear B-cell epitopes, and one conformational B-cell epitope in BALB/c mice. Four linear B-cell epitopes were identified from human sera, three of which overlapped mouse B-cell epitopes. Finally, three human T-cell epitopes were detected on the BB protein. One of these T-cell epitopes is common to BALB/c mice and humans and was localized in the region that contains the albumin-binding site. These data are of interest for the optimization of new carrier molecules derived from BB.
机译：大多数常规疫苗由被杀死的生物或纯化的抗原蛋白组成。此类分子通常免疫原性较差，需要与载体蛋白偶联。我们已经确定了一种新的载体分子，BB，它是来自链球菌菌株G148的G蛋白。我们显示，BB与肽或多糖偶联后能够诱导强烈的抗体反应。为了在BB中定位T和B细胞表位并使它们与分子的白蛋白结合区匹配，我们用BB免疫小鼠，进行B和T胃蛋白酶分析，并将结果与​​人血清和人体细胞的胃蛋白酶进行比较。我们的结果表明，在BALB / c小鼠中，BB具有两个不同的T辅助表位，七个线性B细胞表位和一个构象B细胞表位。从人血清中鉴定出四个线性B细胞表位，其中三个与小鼠B细胞表位重叠。最后，在BB蛋白上检测到三个人类T细胞表位。这些T细胞表位之一是BALB / c小鼠和人类共有的，位于包含白蛋白结合位点的区域。这些数据对于优化衍生自BB的新载体分子很有用。

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