...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Detection of bacterial pyrogens on the basis of their effects on gamma interferon-mediated formation of neopterin or nitrite in cultured monocyte cell lines.
【24h】

Detection of bacterial pyrogens on the basis of their effects on gamma interferon-mediated formation of neopterin or nitrite in cultured monocyte cell lines.

机译：根据细菌热原对培养的单核细胞系中γ干扰素介导的新蝶呤或亚硝酸盐形成的影响来检测细菌热原。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In a number of mammalian cell types, pteridine biosynthesis from guanosine 5'-triphosphate and formation of nitric oxide from L-arginine are induced by gamma interferon (IFN-gamma) and bacterial lipopolysaccharide (LPS). We assessed the possibility of using such metabolic alterations for the in vitro detection of pyrogens. Products from gram-negative and gram-positive bacteria and related synthetic compounds were tested for their potential to induce either of these pathways. Stimulation of pteridine biosynthesis was monitored as the formation of neopterin in the human myelomonocytic cell line THP-1. The formation of nitric oxide was determined as nitrite in murine J774A.1 macrophage cultures. The substances tested included toxic and detoxified parts of LPS and lipid A from Escherichia coli, Salmonella typhimurium, Salmonella minnesota, and Klebsiella pneumoniae as well as lipoteichoic acid and toxic shock syndrome toxin 1 from Staphylococcus aureus. Furthermore, two cell wall compounds from Mycobacterium tuberculosis, trehalose 6,6'-dimycolate and N-acetylmuramyl-L-alanyl-D-isoglutamine, which are active components of Freund's adjuvant, were used. When applied as a single stimulus, only the whole LPS molecule potently stimulated neopterin or nitrite formation. Lipid A and products from gram-positive bacteria were weakly active. For neopterin formation, lipid A required the presence of fetal calf serum. Besides detoxified LPS and independently from the presence of serum, all bacterial compounds tested strongly increased the effects mediated by IFN-gamma. Our results show that bacterial pyrogens can be detected by monitoring the formation of neopterin or nitrite. This may provide a basis for the development of an in vitro assay for the detection of pyrogenic contamination with the aim of replacing the currently used animal test.
机译：在许多哺乳动物细胞类型中，γ干扰素（IFN-γ）和细菌脂多糖（LPS）诱导了鸟苷5'-三磷酸的蝶啶生物合成和L-精氨酸的一氧化氮形成。我们评估了使用这种代谢改变体外检测热原的可能性。测试了革兰氏阴性和革兰氏阳性细菌及相关合成化合物的产物诱导这些途径中任一种的潜力。监测蝶呤生物合成的刺激为人骨髓单核细胞系THP-1中新蝶呤的形成。一氧化氮的形成被确定为鼠类J774A.1巨噬细胞培养物中的亚硝酸盐。测试的物质包括来自大肠杆菌，鼠伤寒沙门氏菌，明尼苏达沙门氏菌和肺炎克雷伯菌的LPS和脂质A的有毒和排毒部分，以及来自金黄色葡萄球菌的脂磷壁酸和毒性休克综合征毒素1。此外，使用了来自结核分枝杆菌的两种细胞壁化合物，海藻糖6，6′-二霉酸酯和N-乙酰村mura基-L-丙氨酰基-D-异谷氨酰胺，它们是弗氏佐剂的活性成分。当作为单一刺激物使用时，只有整个LPS分子才能有效刺激新蝶呤或亚硝酸盐的形成。脂质A和革兰氏阳性细菌的产物活性较弱。对于新蝶呤的形成，脂质A需要存在胎牛血清。除了解毒的LPS并独立于血清之外，所有测试的细菌化合物都强烈增强了IFN-γ介导的作用。我们的结果表明，可以通过监测新蝶呤或亚硝酸盐的形成来检测细菌热原。这可以为开发用于检测热原污染的体外测定法提供基础，以取代当前使用的动物试验。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号