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首页> 外文期刊>Cytotechnology >Effect of some constituents of chicken egg yolk lipoprotein on the growth and IgM production of human-human hybridoma cells and other human-derived cells
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Effect of some constituents of chicken egg yolk lipoprotein on the growth and IgM production of human-human hybridoma cells and other human-derived cells

机译：鸡卵黄脂蛋白某些成分对人-人杂交瘤细胞和其他人源性细胞生长和IgM产生的影响

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摘要

Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-rich fractions) were found to enhance the growth and IgM secretion of HB4C5 cells. The promoting activity was found in the commercial LDL. The lipid fraction in YP-2 fraction conjugated with 2-maltosyl-a-cyclodextrin was found to enhance the growth and IgM secretion of HB4C5 cells. Livetin-rich YP-3 and YP-4 fractions had no significant promoting activity. Commercial γ-livetin and phosphatidyl choline possessed no growth-promoting activity. Phosphatidyl choline enhanced the IgM secretion of HB4C5 cells.
机译：将鸡卵黄脂蛋白（YLP）部分分离成一些成分，并研究了YLP成分对在无血清培养基中培养的HB4C5人-人杂交瘤细胞系生长和免疫球蛋白（IgM和IgG）分泌的影响。在这些级分中，发现YP-1和YP-2级分（富含LDL的级分）可增强HB4C5细胞的生长和IgM分泌。在商业LDL中发现了促进活性。发现与2-麦芽糖基-α-环糊精缀合的YP-2级分中的脂质级分可增强HB4C5细胞的生长和IgM分泌。富含活素的YP-3和YP-4组分没有明显的促进活性。市售的γ-活性蛋白和磷脂酰胆碱不具有促进生长的活性。磷脂酰胆碱增强了HB4C5细胞的IgM分泌。

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