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首页> 外文期刊>Advances in Environmental Technology >Bioremediation of lignocellulosic wastes of food industries by Aspergillus flavus as food and feed additive protein by solid-state fermentation process
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Bioremediation of lignocellulosic wastes of food industries by Aspergillus flavus as food and feed additive protein by solid-state fermentation process

机译：黄曲霉作为食品和饲料添加剂蛋白的固态发酵工艺对食品工业木质纤维素废物的生物修复

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摘要

The lignocellulosic wastes produced in food industries are suitable raw materials for the production of biological products. In this study, the solid state fermentation of Aspergillus flavus on lignocellulosic wastes was evaluated for microbial protein production. The fraction of the full factorial method was applied for experiment design and process optimization. The results analysis was performed through signal to noise statistical index using the Taguchi approach via Qualitek-4 software. Glycine, ammonium sulfate and iron sulfate concentration as well as temperature were considered as effective parameters. The maximum biomass concentration of 45.7 g/kg containing 55.75% (w/w) pure protein was obtained at optimal conditions including 0.5, 0.02, and 2 g/kg of ammonium sulfate, iron sulfate and glycine, respectively, at 25 °C. Ammonium sulfate (33.78% (w/w) contribution) and culture temperature (31.98% contribution) were evaluated as the most effective factors on biomass and microbial protein production. The highest interaction occurred between ammonium sulfate and glycine with an interaction severity index of 50.03%. The low deviation of 3.94% was determined between optimum theoretical biomass concentration (43.9 g/kg) and the experimentally measured one (45.7 g/kg). Due to the high protein content of 55.75% (w/w), Aspergillus flavus was introduced as a suitable strain for industrial protein production.
机译：食品工业中产生的木质纤维素废物是生产生物产品的合适原料。在这项研究中，评估了曲霉菌在木质纤维素废料上的固态发酵的微生物蛋白生产。全阶乘方法的一部分用于实验设计和过程优化。结果分析是通过Qualitek-4软件使用Taguchi方法通过信噪比统计指标进行的。甘氨酸，硫酸铵和硫酸铁的浓度以及温度被认为是有效参数。在最佳条件下，在25°C下分别获得0.5、0.02和2 g / kg的硫酸铵，硫酸铁和甘氨酸的最佳生物量浓度为45.7 g / kg，其中包含55.75（w / w）纯蛋白。评估硫酸铵（贡献率为33.78％（w / w））和培养温度（贡献率为31.98％）是影响生物量和微生物蛋白质生产的最有效因素。硫酸铵与甘氨酸之间的相互作用最大，相互作用严重性指数为50.03％。在最佳理论生物量浓度（43.9 g / kg）和实验测量值（45.7 g / kg）之间确定了3.94％的低偏差。由于蛋白质含量高达55.75％（w / w），因此引入了黄曲霉作为适合工业蛋白质生产的菌株。

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