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An efficient and rapid method for protein detection with an example of sulfide-quinone reductase expressed in Escherichia coli

机译:一种高效,快速的蛋白质检测方法,以在大肠杆菌中表达的硫化物-醌还原酶为例

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Conventional Coomassie brilliant blue (CBB) staining is widely used in protein detection as it is inexpensive and reproducible. Conversely, it is also very?time-consuming?and cumbersome. In this study, CBB(R-250)-microwaved-water (CMW) was used to detect the sulfide-quinone reductase (SQR) protein expressed inEsherichia coli?by staining and destaining in under 30 min. The CMW method has similar detection capabilities as the conventional CBB method. However, compared with the CBB protocol, it did not produce pungent odors, and was more efficient and rapid, which saved more than 4 to 10 h. This method is an acceptable alternative for the preliminary detection of protein expression.
机译:传统的考马斯亮蓝(CBB)染色技术便宜且可重复,因此广泛用于蛋白质检测。相反,它也非常“耗时”且麻烦。在这项研究中,使用CBB(R-250)-微波水(CMW)通过染色和脱色在30分钟内检测大肠杆菌中表达的硫化物-醌还原酶(SQR)蛋白。 CMW方法具有与常规CBB方法相似的检测能力。但是,与CBB协议相比,它不会产生刺激性气味,并且效率更高,速度更快,可节省4至10个小时以上。该方法是初步检测蛋白质表达的可接受方法。

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