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首页> 外文期刊>African Journal of Biotechnology >Biosurfactant production by Bacillus subtilis UFPEDA 86 using papaya (Carica papaya L.) waste as substrate: Viability studies and pH influence of the culture medium
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Biosurfactant production by Bacillus subtilis UFPEDA 86 using papaya (Carica papaya L.) waste as substrate: Viability studies and pH influence of the culture medium

机译：枯草芽孢杆菌UFPEDA 86以木瓜（Carica papaya L.）废物为底物生产生物表面活性剂：培养基的活力研究和pH影响

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Biosurfactants are surface-active compounds derived from microorganisms and offer several advantages over chemical surfactants, such as low toxicity, good biodegradability and ecological acceptability. Even though interest in biosurfactants is increasing, these bioproducts do not compete economically with synthetic surfactants due to the overall costs of the bioprocess. The use of inexpensive raw materials is an important approach to reduce these costs since the substrate price account for 10 to 30% of the final product expenses. In this study, papaya (Carica papaya L.) waste was used as a substrate to produce biosurfactant by Bacillus subtilis UFPEDA 86 strain. In addition, culture medium pH was corrected from 5.25 to 6.8 in order to analyze the influence of this variable on the biosurfactant production. The submerged fermentation was carried out on a shaker incubator at 37°C, 200 rpm for 96 h. Biomass and substrate concentration, surface tension, emulsification index and critical micelle concentration were analyzed. The strain was well adapted to both substrates studied, without and with pH correction. Using the broth without pH correction (pH=5.25), a maximum cell concentration of 1.07 g L-1 at 36 h a 25.5% surface tension reduction, emulsification index of around 61% and critical micelle concentration of about 35 mg L-1 was obtained. Using the broth with pH correction to 6.8, better results; maximum cell concentration of 1.14 g L-1 at 24 h, a 32.5% surface tension reduction, emulsification index of around 66% and critical micelle concentration at about 35 mg L-1 was obtained. The papaya waste proved to be an effective substrate in the biosurfactant production by B. subtilis UFPEDA 86 and the pH variable proved to be of great importance in the yield of this process.
机译：生物表面活性剂是衍生自微生物的表面活性化合物，与化学表面活性剂相比具有许多优势，例如低毒性，良好的生物降解性和生态可接受性。即使对生物表面活性剂的兴趣在增加，但由于生物过程的总成本，这些生物产品仍无法与合成表面活性剂经济竞争。使用廉价的原材料是降低这些成本的重要方法，因为基板价格占最终产品费用的10％至30％。在这项研究中，木瓜（Carica papaya L.）废物被用作底物，通过枯草芽孢杆菌UFPEDA 86菌株生产生物表面活性剂。另外，将培养基的pH值从5.25校正为6.8，以分析该变量对生物表面活性剂生产的影响。在37℃，200rpm的摇床培养箱中进行浸没发酵96小时。分析了生物质和底物浓度，表面张力，乳化指数和临界胶束浓度。该菌株很好地适应了所研究的两种底物，无需校正pH。使用未经pH校正的肉汤（pH = 5.25），在36公顷时最大细胞浓度为1.07 g L-1，表面张力降低25.5％，乳化指数约为61％，临界胶束浓度约为35 mg L-1。 。使用pH校正至6.8的肉汤可获得更好的效果;获得的最大细胞浓度为24小时时的1.14 g L-1、32.5％的表面张力降低，约66％的乳化指数和约35 mg L-1的临界胶束浓度。木瓜废料被证明是枯草芽孢杆菌UFPEDA 86生产生物表面活性剂的有效底物，并且pH值被证明对该方法的收率非常重要。

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