• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Indian Journal of Virology >Biological and Molecular Characterization of Two Distinct Tomato Strains of Cucumber mosaic virus Based on Complete RNA-3 Genome and Subgroup Specific Diagnosis
【24h】

Biological and Molecular Characterization of Two Distinct Tomato Strains of Cucumber mosaic virus Based on Complete RNA-3 Genome and Subgroup Specific Diagnosis

机译：基于完全RNA-3基因组和亚组特异性诊断的两个黄瓜花叶病毒不同菌株的生物学和分子表征

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cucumber mosaic virus (CMV) is one of the most important viral pathogen infecting several plant species in India. Five isolates of CMV obtained from cucumber, muskmelon, tobacco and tomato from distinct geographical locations in India were analysed based on host-reactions and genome sequence. The majority of the isolates were very similar and only two isolates, Tfr-In and Tss-In showed distinct symptoms in tomato and high sequence diversity (77.8%) in coat protein (CP) gene. Tfr-In was isolated from tomato fruit showing grey patches in Aurangabad and Tss-In from tomato plant showing shoe-string symptoms in New Delhi. The RNA-3 genomes of Tfr-In (2,214 nt; {"type":"entrez-nucleotide","attrs":{"text":"JF279606","term_id":"340774919","term_text":"JF279606"}}JF279606), shared only 70.3% nucleotide sequence identity with Tss-In (2,178 nt; {"type":"entrez-nucleotide","attrs":{"text":"JF279605","term_id":"340774916","term_text":"JF279605"}}JF279605. The complete RNA-3 genome of Tss-In and Tfr-In were compared with that of 65 CMV isolates reported from various plants of the world, which formed four distinct subclades-IA, -IB, -IC and -II. The Tfr-In isolate clustered with the CMV subgroup-IB and Tss-In with the subgroup-II. The comparison of the RNA-3 sequence of both the isolates revealed maximum heterogeneity in the intergenic region (IR). Reverse transcriptase polymerase chain reaction (RT-PCR) based detection of CMV subgroup-I and -II was developed designing primers from flanking IR region. The specificity of the RT-PCR detection was confirmed using Tfr-In and Tss-In representing subgroup-I and -II and validated with field samples of tomato, cucurbits and chilli. This is the first report of complete RNA-3 of subgroup-IB CMV causing grey patches in tomato fruit and subgroup-II CMV causing shoe-string symptoms in tomato in India. The present and previous studies together showed that tomato in India was affected by multiple strains of CMV.
机译：黄瓜花叶病毒（CMV）是感染印度几种植物的最重要的病毒病原体之一。根据宿主反应和基因组序列分析了从印度不同地理位置的黄瓜，甜瓜，烟草和番茄中获得的5种CMV分离株。大多数分离株非常相似，只有两个分离株，Tfr-In和Tss-In在番茄中表现出明显的症状，并且在外壳蛋白（CP）基因中具有很高的序列多样性（77.8％）。 Tfr-In是从在奥兰加巴德呈灰色斑块的番茄果实中分离出来的，而Tss-In是从在新德里表现出鞋带状症状的番茄植物中分离出来的。 Tfr-In（2,214 nt; {“ type”：“ entrez-nucleotide”，“ attrs”：{“ text”：“ JF279606”，“ term_id”：“ 340774919”，“ term_text”：“的RNA-3基因组JF279606“}} JF279606）与Tss-In（2,178 nt; {” type“：” entrez-nucleotide“，” attrs“：{” text“：” JF279605“，” term_id“）仅共享70.3％的核苷酸序列同一性： “ 340774916”，“ term_text”：“ JF279605”}} JF279605。将Tss-In和Tfr-In的完整RNA-3基因组与世界上不同植物报道的65个CMV分离株的RNA-3基因组进行了比较，形成了四个不同的分支-IA，-IB，-IC和-II。Tfr-In分离株与CMV亚组IB聚在一起，Tss-In与II亚组聚类，两种分离株的RNA-3序列比较显示最大异质性。开发了基于逆转录酶聚合酶链反应（RT-PCR）的CMV-I和-II亚型检测方法，设计了侧翼IR区的引物，并用Tfr-In确定了RT-PCR检测的特异性和Tss-In代表子组I和-II，以及已通过番茄，葫芦和辣椒田间样品验证。这是在印度，IB亚类CMV完整RNA-3导致番茄果实出现灰色斑块的完整报道，而II II CMV亚类引起印度番茄的鞋带症状的首次报道。目前和以前的研究一起表明，印度的番茄受到多种CMV菌株的影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号