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首页> 外文期刊>Romanian Journal of Biophysics >CHOLESTEROL EXCHANGE BETWEEN PHOSPHOLIPID VESICLES
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CHOLESTEROL EXCHANGE BETWEEN PHOSPHOLIPID VESICLES

机译：磷脂囊泡之间的胆固醇交换

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摘要

The mechanism of cholesterol exchange has been investigated by following the transfer of radiolabeled cholesterol from negatively charged, unilamellar donor vesicles to neutral acceptor vesicles. Donor and acceptor vesicles were incubated together and were stable to fusion over the course of the experiment. At intervals, donor and acceptor vesicles were separated by passage through a column of DEAE-Sepharose. Ion exchange chromatography was used for rapid separation of donor and acceptor (present in 10-fold molar excess) vesicles. The kinetics of [14C] cholesterol transfer between unilamellar vesicles was described and analyzed in the presence of sucrose buffer; 10% FCS (fetal calf serum) and 50% FCS. The process is first order kinetics. This means that acceleration of the off-rate must be due to donor-acceptor interactions at short distances. It was found that the presence of serum in the medium greatly diminished rather than increased the total transfer of radiolabelled cholesterol from donor vesicles to the acceptor vesicles.
机译：已经通过追踪放射性标记的胆固醇从带负电荷的单层供体囊泡向中性受体囊泡的转移来研究胆固醇交换的机制。供体和受体囊泡一起孵育，在整个实验过程中对融合稳定。每隔一定时间，通过DEAE-Sepharose柱分离供体和受体囊泡。离子交换色谱法用于快速分离供体和受体（摩尔过量10倍）的囊泡。描述了在蔗糖缓冲液存在下[14C]胆固醇在单层囊泡之间转移的动力学。 10％FCS（胎牛血清）和50％FCS。该过程是一阶动力学。这意味着不合格率的加速必须归因于短距离上的供体-受体相互作用。已经发现，培养基中血清的存在大大减少而不是增加了放射性标记的胆固醇从供体囊泡向受体囊泡的总转移。

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