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首页> 外文期刊>Journal of medical bacteriology. >Identification and Extraction of Chicken Egg Yolk Immunoglobulin from Egg by Polyethylene Glycol (PEG) Precipitation
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Identification and Extraction of Chicken Egg Yolk Immunoglobulin from Egg by Polyethylene Glycol (PEG) Precipitation

机译：聚乙二醇（PEG）沉淀法鉴定和提取鸡蛋中的蛋黄免疫球蛋白

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Background: Staphylococcus aureus strains exhibiting multiple antibiotic resistances are isolated from most communities and hospital infections. Treatment of patients with these infections has been difficult. The aim of this study was to detect and extract, the egg yolk immunoglobulin Y as a potential source of anti- S. aureus antibody.?Methods: Specific IgY was produced by immunizing hens with formalin-killed S. aureus. The specificity of serum`s antibody was confirmed by ELISA method. The antibodies were extracted from egg yolk by polyethylene glycol (PEG) precipitation. Proteins were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).?Results: Chicken egg yolk antibodies (IgY) were raised against S. aureus in the serum after injections. Up to 104 dilution specific antibodies were determined in serum.?Conclusion: The results of the ELISA indicates the specificity of the immunoglobulin Y to the target antigen. In order to find a viable alternative to antibiotic treatments, more research must be done on the ability of these antibodies to inhibit the growth of S. aureus.
机译：背景：从大多数社区和医院感染中分离出具有多种抗生素耐药性的金黄色葡萄球菌菌株。患有这些感染的患者的治疗一直很困难。这项研究的目的是检测和提取蛋黄免疫球蛋白Y作为抗金黄色葡萄球菌抗体的潜在来源。方法：通过用福尔马林杀死的金黄色葡萄球菌免疫母鸡来产生特异性IgY。血清抗体的特异性通过ELISA法确定。通过聚乙二醇（PEG）沉淀从蛋黄中提取抗体。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析蛋白质。结果：注射后血清中产生了针对金黄色葡萄球菌的鸡卵黄抗体（IgY）。血清中最多可检测104种稀释特异性抗体。结论：ELISA结果表明免疫球蛋白Y对靶抗原的特异性。为了找到可行的替代抗生素治疗方法，必须对这些抗体抑制金黄色葡萄球菌生长的能力进行更多的研究。

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