The Specific Genes for Lantibiotic Mutacin II Biosynthesis in Streptococcus mutans T8 Are Clustered and Can Be Transferred En Bloc

Ping Chen; FengXia Qi; Jan Novak; Page W. Caufield

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The Specific Genes for Lantibiotic Mutacin II Biosynthesis in Streptococcus mutans T8 Are Clustered and Can Be Transferred En Bloc

机译：变形链球菌T8中羊毛硫抗生素突变蛋白II生物合成的特定基因是聚集的，可以整批转移。

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Mutacin II is a ribosomally synthesized peptide lantibiotic produced by group II Streptococcus mutans. DNA sequencing has revealed that the mutacin II biosynthetic gene cluster consists of seven specific open reading frames: a regulator (mutR), the prepromutacin structural gene (mutA), a modifying protein (mutM), an ABC transporter (mutT), and an immunity cluster (mutFEG). Transformations of a non-mutacin-producing strain, S. mutans UA159, and a mutacin I-producing strain, S. mutans UA140, with chromosomal DNA from S. mutans T8 with anaphIII marker inserted upstream of the mutacin II structural gene yielded transformants producing mutacin II and mutacins I and II, respectively.

机译：Mutacin II是由II组变形链球菌产生的核糖体合成肽羊毛硫抗生素。 DNA测序表明，诱变蛋白II生物合成基因簇由七个特定的开放阅读框组成：调节剂（mutR），前promutacin结构基因（mutA），修饰蛋白（mutM），ABC转运蛋白（mutT）和免疫力集群（mutFEG）。用来自诱变链球菌T8的染色体DNA插入带有诱变蛋白II结构基因上游的anaphIII标记，转化不产生诱变酶的菌株S. mutans UA159和产生诱变蛋白I的菌株S. mutans UA140。突变蛋白II和突变蛋白I和II。

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《Applied and Environmental Microbiology》 |1999年第3期|共5页
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Ping Chen; FengXia Qi; Jan Novak; Page W. Caufield;
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1. The Specific Genes for Lantibiotic Mutacin II Biosynthesis in Streptococcus mutans T8 Are Clustered and Can Be Transferred En Bloc [J] . Ping Chen, FengXia Qi, Jan Novak, Applied and Environmental Microbiology . 1999,第3期

机译：变形链球菌T8中羊毛硫抗生素突变蛋白II生物合成的特定基因是聚集的，可以整批转移。
2. Structure-Activity Study of the Lantibiotic Mutacin II from Streptococcus mutans T8 by a Gene Replacement Strategy [J] . Ping Chen, Jan Novak, Marion Kirk, Applied and Environmental Microbiology . 1998,第7期

机译：变形链球菌T8的羊毛硫抗生素突变蛋白II的结构活性研究通过基因替代策略。
3. Nucleotide and deduced amino acid sequences of the lacR, lacABCD, and lacFE genes encoding the repressor, tagatose 6-phosphate gene cluster, and sugar-specific phosphotransferase system components of the lactose operon of Streptococcus mutans. [J] . E L Rosey, G C Stewart Journal of bacteriology . 1992,第19期

机译：lacR，lacABCD和lacFE基因的核苷酸序列和推导的氨基酸序列，它们编码变形链球菌乳糖操纵子的阻遏物，塔格糖6-磷酸基因簇和糖特异性磷酸转移酶系统组分。
4. STREPTOCOCCUS MACEDONICUS PRODUCES THE LANTIBIOTIC MACEDOCIN WHEN GROWN IN MILK FROM BIOSYNTHESIS TO APPLICATIONS [C] . TSAKALIDOU Effie, ANASTASIOU Rania, PAPADELLI Marina, Microbial diversity 2011 : environmental stress and adaptation . 2011

机译：当牛奶在从生物合成到应用的过程中生长时，链球菌可生产出乳酸菌马其顿素
5. Mutacin IV production in Streptococcus mutans UA159: Characterization, regulation, and mechanism of self-immunity [D] . Hossain, Mohammad Shahnoor 2012

机译：变形链球菌UA159中突变蛋白IV的产生：自身免疫的表征，调控和机制
6. The Specific Genes for Lantibiotic Mutacin II Biosynthesis in Streptococcus mutans T8 Are Clustered and Can Be Transferred En Bloc [O] . Ping Chen, FengXia Qi, Jan Novak, 1999

机译：变形链球菌T8中羊毛硫抗生素突变蛋白II生物合成的特定基因是群集的可以整批转移。
7. The Specific Genes for Lantibiotic Mutacin II Biosynthesis in Streptococcus mutans T8 Are Clustered and Can Be Transferred En Bloc [O] . Ping Chen, FengXia Qi, Jan Novak, 1999

机译：在链球菌变形T8中的毫耳菌变异II生物合成的特定基因是聚集的，可以转移en Bloc

The Specific Genes for Lantibiotic Mutacin II Biosynthesis in Streptococcus mutans T8 Are Clustered and Can Be Transferred En Bloc

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