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Particulate Fractions of Neisseria gonorrhoeae

机译:淋病奈瑟氏球菌的颗粒级分

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Passage of gonococcal cells through a Ribi cell fractionator produced a mixture of protoplasm and cell particulates, the latter being separable in sucrose solutions. Separation of these particulates resulted in the detection of objects identified as cell walls and “plasts.” The latter objects had an average diameter of 1.00 ± 0.05 μm and consisted of spatially oriented granules surrounded by a membrane-like structure only. Cell wall structures were not observed with these plasts. Plasts were morphologically stable in distilled water and at low pH values. Although 50% more pressure was required to rupture plasts than whole cells, plast morphology was destroyed by organic solvents. Whole cell and plast morphology was examined by electron and phase microscopy, and certain correlations were made between structure and morphological stability. Cell wall antiserum reactivities were quantitatively different for plasts and cell walls. Serological reactivities of plasts were separable by sonic oscillation and differential centrifugation of the sonically treated material.
机译:淋球菌细胞通过Ribi细胞分离器产生了原生质和细胞颗粒的混合物,后者可以在蔗糖溶液中分离。这些微粒的分离导致检测到被识别为细胞壁和“塑料”的物体。后面的物体的平均直径为1.00±0.05μm,由仅被膜状结构包围的空间定向颗粒组成。这些塑料没有观察到细胞壁结构。塑性体在蒸馏水中形态稳定, p H值低。尽管破裂塑料所需的压力要比整个细胞高50%,但塑料的形态却被有机溶剂破坏了。通过电子和相显微镜检查全细胞和塑料的形态,并在结构和形态稳定性之间建立了一定的联系。质体和细胞壁的细胞壁抗血清反应性在数量上是不同的。通过超声处理的材料的声波振荡和离心分离可以分离出塑料的血清反应性。

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