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首页> 外文期刊>Infection and immunity >Assessment of attachment of Neisseria gonorrhoeae to HeLa cells by double radiolabeling.
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Assessment of attachment of Neisseria gonorrhoeae to HeLa cells by double radiolabeling.

机译：通过双重放射标记评估淋病奈瑟氏球菌对HeLa细胞的附着。

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Attachment of Neisseria gonorrhoeae to HeLa cells was assessed by a technique using double radioisotopic labeling. Piliated, virulent bacteria from colony type 2 attached to HeLa cells to a greater extent than nonpiliated, avirulent bacteria from colony type 4. Maximal attachment rates for bacteria from both colony types occurred during the early incubation periods at 37 degrees C, and the HeLa cells appeared saturated at 4 h. Attachment was maximum at pH 6.5 and dependent upon the multiplicity of infection. Treatment of the HeLa cells with trypsin diminished the degree of attachment, but this effect substantially disappeared by 24 h after trypsin treatment. Scanning electron microscopy revealed bacteria of colony types 2 and 4 adhered to the HeLa cell surface. Thin-section transmission electron microscopy showed that bacteria were associated with the surface of the HeLa cell but not ingested.
机译：通过使用双重放射性同位素标记的技术评估淋病奈瑟氏球菌对HeLa细胞的附着。来自第2个菌落的有毒力的细菌比第4个菌落的无毒力的无毒细菌附着的程度更大。这两种菌落的细菌在37°C的早期孵化期以及HeLa细胞的最大附着率都达到了在4 h出现饱和。在pH 6.5时，附着力最大，这取决于感染的多重性。用胰蛋白酶处理HeLa细胞可减少附着程度，但在胰蛋白酶处理后24小时该作用基本消失。扫描电子显微镜显示，菌落类型2和4的细菌粘附在HeLa细胞表面。薄层透射电子显微镜显示细菌与HeLa细胞表面相关，但未被摄入。

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