• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Mycoplasma-dependent activation of normal mouse lymphocytes: requirement for functional T lymphocytes in the cytotoxicity reaction mediated by Mycoplasma arthritidis.
【24h】

Mycoplasma-dependent activation of normal mouse lymphocytes: requirement for functional T lymphocytes in the cytotoxicity reaction mediated by Mycoplasma arthritidis.

机译：正常小鼠淋巴细胞的支原体依赖性激活：由关节炎支原体介导的细胞毒性反应中功能性T淋巴细胞的需求。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Syngeneic and allogeneic target cells were killed in the presence of CBA mouse lymphocytes and viable Mycoplasma arthritidis. Medium supplementation had no effect on the response. Nonviable M. arthritidis was also capable of stimulating lymphocytotoxicity, although to a much lesser extent. Cytotoxicity was shown to be largely dependent upon the lymphocytes, since lymphocytes preincubated with mycoplasmas and treated to remove remaining organisms were highly toxic to target cells, whereas supernatants prepared from lymphocyte/mycoplasma mixtures exhibited minimal effects. A 6-h exposure of lymphocytes to mycoplasmas at a ratio of 100:1 was sufficient for commitment to target cell killing. Functional lymphocytes were required for the reaction, since gamma-irradiated lymphocytes did not develop cytotoxic potential despite the fact that the mycoplasmas replicated equally well in the presence of these and untreated lymphocytes. Furthermore, lymphocytes already activated with mycoplasmas lost cytotoxic potential after disruption. The kinetics and degree of lymphocytotoxicity induced by M. arthritidis and phytohemagglutinin toward 51Cr-labeled syngeneic fibroblasts were similar. Removal of most B cells and other adherent cells by column separation did not abrogate the cytotoxic effect. Lymphocyte suspensions treated with anti-Thy 1 antiserum and complement exhibited a marked decrease in their cytotoxic potential when added to labeled target cells in the presence of M. arthritidis. We conclude that the cytotoxic reaction is dependent upon the T-lymphocyte subpopulation.
机译：同种和同种异体靶细胞在CBA小鼠淋巴细胞和活的支原体关节炎的存在下被杀死。中度补充对反应没有影响。无生命的M. arthritidis也能够刺激淋巴细胞毒性，尽管程度要小得多。已显示细胞毒性在很大程度上取决于淋巴细胞，因为与支原体预孵育并经过处理以去除残留生物的淋巴细胞对靶细胞具有高毒性，而由淋巴细胞/支原体混合物制备的上清液则表现出最小的作用。淋巴细胞以100：1的比例暴露于支原体6小时，足以杀死靶细胞。反应需要功能性淋巴细胞，因为尽管在这些和未处理的淋巴细胞存在下支原体均能很好地复制，但伽马射线照射的淋巴细胞并没有产生细胞毒性潜能。此外，已经被支原体激活的淋巴细胞在破坏后丧失了细胞毒性潜力。关节炎分枝杆菌和植物血凝素对51Cr标记的同基因成纤维细胞诱导的淋巴细胞毒性的动力学和程度相似。通过柱分离除去大多数B细胞和其他贴壁细胞没有消除细胞毒性作用。用抗Thy 1抗血清和补体处理的淋巴细胞悬液，在有节肢炎支原体存在的情况下添加到标记的靶细胞时，其细胞毒性潜力显着降低。我们得出结论，细胞毒性反应取决于T淋巴细胞亚群。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号