• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Cloning and Characterization of vuuA, a Gene Encoding the Vibrio vulnificus Ferric Vulnibactin Receptor
【24h】

Cloning and Characterization of vuuA, a Gene Encoding the Vibrio vulnificus Ferric Vulnibactin Receptor

机译：vuuA的克隆和鉴定，vuuA是一个编码弧菌弧菌铁vulnibactin受体的基因

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The ability of Vibrio vulnificus to acquire iron from the host has been shown to correlate with virulence. Many iron transport genes are regulated by iron, and in V. vulnificus, transcriptional regulation by iron depends on thefur gene. The N-terminal amino acid sequence of a 72-kDa iron-regulated outer membrane protein purified from a V. vulnificus fur mutant had 53% homology with the first 15 amino acids of the mature protein of the Vibrio choleraevibriobactin receptor, ViuA. In this report, we describe the cloning, DNA sequence, mutagenesis, and analysis of transcriptional regulation of the structural gene for VuuA, the vulnibactin receptor of V. vulnificus. Analysis of the DNA sequence of the vuuApromoter region demonstrated a sequence identical to the upstream Fur box of V. cholerae viuA. Northern blot analysis showed that the transcript was strongly regulated by iron. The amino acid sequence of VuuA was 74% identical to the sequence of V. choleraeViuA and was homologous to those of several TonB-dependent outer membrane receptors. An internal deletion of the V. vulnificus vuuA gene resulted in the loss of expression of the 72-kDa protein and the loss of the ability to use transferrin or vulnibactin as a source of iron. This mutant showed reduced virulence in an infant mouse model. Introduction of a plasmid containing the completeviuA coding sequence and 342 bp of upstream DNA into the mutant restored ferric vulnibactin and ferric transferrin utilization to the mutant.
机译：创伤弧菌从宿主获得铁的能力已显示与毒力相关。许多铁转运基因受铁调节，而在V. vulnificus中，铁的转录调节取决于糠醛基因。从V. vulnificus fur突变体纯化的72 kDa铁调节的外膜蛋白的N末端氨基酸序列与霍乱弧菌细菌VibrA受体ViuA的成熟蛋白的前15个氨基酸具有53％的同源性。在此报告中，我们描述了V. vulnificus的vulnibactin受体VuuA的结构基因的克隆，DNA序列，诱变和转录调控分析。对vuuA启动子区域的DNA序列的分析表明，该序列与霍乱弧菌viuA的上游Fur盒相同。 Northern印迹分析表明，转录物受铁强烈调节。 VuuA的氨基酸序列与霍乱弧菌ViuA的序列具有74％的同一性，并且与几种TonB依赖性外膜受体的氨基酸序列同源。 V. vulnificus vuuA基因的内部缺失导致72 kDa蛋白表达的损失以及使用转铁蛋白或vulnibactin作为铁源的能力的丧失。该突变体在婴儿小鼠模型中显示出降低的毒力。将含有完整viuA编码序列和342 bp上游DNA的质粒导入突变体中，可恢复铁vulnibactin和铁转铁蛋白对突变体的利用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号