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首页> 外文期刊>Infection and immunity >Porphyromonas gingivalis Traffics to Autophagosomes in Human Coronary Artery Endothelial Cells
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Porphyromonas gingivalis Traffics to Autophagosomes in Human Coronary Artery Endothelial Cells

机译：牙龈卟啉单胞菌运输到人冠状动脉内皮细胞中的自噬体。

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Porphyromonas gingivalis is a periodontal pathogen that also localizes to atherosclerotic plaques. Our previous studies demonstrated that P. gingivalis is capable of invading endothelial cells and that intracellular bacteria are contained in vacuoles that resemble autophagosomes. In this study, we have examined the trafficking of P. gingivalis 381 to the autophagic pathway. P. gingivalis 381 internalized by human coronary artery endothelial (HCAE) cells is located within vacuoles morphologically identical to autophagosomes. The progression ofP. gingivalis 381 through intracellular vacuoles was analyzed by immunofluorescence microscopy. Vacuoles containingP. gingivalis colocalize with Rab5 and HsGsa7p early after internalization. At later times, P. gingivaliscolocalizes with BiP and then progresses to a vacuole that contains BiP and lysosomal glycoprotein 120. Late endosomal markers and the lysosomal cathepsin L do not colocalize with P. gingivalis 381. The intracellular survival of P. gingivalis 381 decreases over 8 h in HCAE cells pretreated with the autophagy inhibitors 3-methyladenine and wortmannin. In addition, the vacuole containing P. gingivalis 381 lacks BiP but contains cathepsin L in the presence of wortmannin. These results suggest that P. gingivalis 381 evades the endocytic pathway to lysosomes and instead traffics to the autophagosome.
机译：牙龈卟啉单胞菌是一种牙周病原体，也定位于动脉粥样硬化斑块。我们以前的研究表明 P。齿龈炎能够侵入内皮细胞，并且胞内细菌包含在类似于自噬体的液泡中。在这项研究中，我们研究了 P的贩运。齿龈 381进入自噬途径。 P。被人冠状动脉内皮细胞（HCAE）内化的齿龈381位于与自身吞噬体形态相同的液泡中。 P的进展。通过免疫荧光显微镜分析了通过细胞内液泡形成的齿龈381。含 P的蒸气。牙龈炎在内部化后与Rab5和HsGsa7p共定位。在以后的时间， P。 gingivalis 与BiP共同定位，然后发展为包含BiP和溶酶体糖蛋白120的液泡。晚期的内体标记和溶酶体组织蛋白酶L与 P不共定位。牙龈炎 381. P的细胞内存活。在自噬抑制剂3-甲基腺嘌呤和渥曼青霉素预处理的HCAE细胞中，齿龈381减少了8小时。另外，液泡中含有 P。齿龈炎 381缺少BiP，但在渥曼青霉素存在的情况下含有组织蛋白酶L。这些结果表明 P。齿龈 381避开了向溶酶体的内吞途径，而是向自噬体转移。

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