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Glycogen and Maltose Utilization by Escherichia coli O157:H7 in the Mouse Intestine

机译：小鼠肠中大肠杆菌O157：H7的糖原和麦芽糖利用

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Mutant screens and transcriptome studies led us to consider whether the metabolism of glucose polymers, i.e., maltose, maltodextrin, and glycogen, is important for Escherichia coli colonization of the intestine. By using the streptomycin-treated mouse model, we found that catabolism of the disaccharide maltose provides a competitive advantage in vivo to pathogenic E. coli O157:H7 and commensal E. coli K-12, whereas degradation of exogenous forms of the more complex glucose polymer, maltodextrin, does not. The endogenous glucose polymer, glycogen, appears to play an important role in colonization, since mutants that are unable to synthesize or degrade glycogen have significant colonization defects. In support of the hypothesis that E. coli relies on internal carbon stores to maintain colonization during periods of famine, we found that by providing a constant supply of a readily metabolized sugar, i.e., gluconate, in the animal's drinking water, the competitive disadvantage of E. coli glycogen metabolism mutants is rescued. The results suggest that glycogen storage may be widespread in enteric bacteria because it is necessary for maintaining rapid growth in the intestine, where there is intense competition for resources and occasional famine. An important implication of this study is that the sugars used by E. coli are present in limited quantities in the intestine, making endogenous carbon stores valuable. Thus, there may be merit to combating enteric infections by using probiotics or prebiotics to manipulate the intestinal microbiota in such a way as to limit the availability of sugars preferred by E. coli O157:H7 and perhaps other pathogens.
机译：突变筛选和转录组研究使我们考虑了葡萄糖聚合物（即麦芽糖，麦芽糊精和糖原）的代谢对于肠道大肠杆菌定植是否重要。通过使用链霉素处理的小鼠模型，我们发现二糖麦芽糖的分解代谢在体内对致病性 E具有竞争优势。大肠杆菌 O157：H7和常见的 E。大肠杆菌K-12，而降解外源形式的更复杂的葡萄糖聚合物麦芽糊精则不会。内源性葡萄糖聚合物糖原似乎在定植中起着重要作用，因为无法合成或降解糖原的突变体具有明显的定殖缺陷。支持 E的假设。大肠杆菌在饥荒期间依靠内部碳存储来维持定居，我们发现通过在动物的饮用水中持续提供易于代谢的糖（即葡萄糖酸盐），是 E的竞争劣势。挽救了大肠杆菌糖原代谢突变体。结果表明，糖原存储可能在肠细菌中广泛分布，因为对于在激烈竞争资源和偶尔发生饥荒的肠道中维持快速生长是必要的。这项研究的重要意义是 E使用的糖。大肠中存在的大肠杆菌数量有限，因此内源性碳库具有重要价值。因此，通过使用益生菌或益生元来限制肠道菌群的肠道菌群的使用来限制肠道菌群的优势可能是有益的。 O157：H7和其他病原体。

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