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首页> 外文期刊>Infection and immunity >Listeria monocytogenes Flagella Are Used for Motility, Not as Adhesins, To Increase Host Cell Invasion
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Listeria monocytogenes Flagella Are Used for Motility, Not as Adhesins, To Increase Host Cell Invasion

机译：单核细胞增生李斯特氏菌鞭毛用于运动，而不是作为粘附素用于增加宿主细胞的侵袭

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Flagellar structures contribute to the virulence of multiple gastrointestinal pathogens either as the effectors of motility, as adhesins, or as a secretion apparatus for virulence factors. Listeria monocytogenes is a food-borne, gram-positive pathogen that uses flagella to increase the efficiency of epithelial cell invasion (A. Bigot, H. Pagniez, E. Botton, C. Frehel, I. Dubail, C. Jacquet, A. Charbit, and C. Raynaud, Infect. Immun. >73:5530-5539, 2005; L. Dons, E. Eriksson, Y. Jin, M. E. Rottenberg, K. Kristensson, C. N. Larsen, J. Bresciani, and J. E. Olsen, Infect. Immun. >72:3237-3244, 2004). In this study, we aimed to elucidate the mechanism by which flagella contribute to L. monocytogenes invasion. To examine the role of flagella as adhesins, invasion and adhesion assays were performed with flagellated motile and nonmotile bacteria and nonflagellated bacteria. We observed that flagellated but nonmotile bacteria do not adhere to or invade human epithelial cells more efficiently than nonflagellated bacteria. These results indicated that flagella do not function as adhesins to enhance the adhesion of L. monocytogenes to targeted host cells. Instead, it appears that motility is important for tissue culture invasion. Furthermore, we tested whether motility contributes to early colonization of the gastrointestinal tract using a competitive index assay in which mice were infected orally with motile and nonmotile bacteria in a 1:1 ratio. Differential bacterial counts demonstrated that motile bacteria outcompete nonmotile bacteria in the colonization of the intestines at early time points postinfection. This difference is also reflected in invasion of the liver 12 h later, suggesting that flagellum-mediated motility enhances L. monocytogenes infectivity soon after bacterial ingestion in vivo.
机译：鞭毛结构作为运动的影响因子，粘附素或作为致病因子的分泌设备，对多种胃肠道病原体的致病力作出贡献。 单核细胞增生李斯特菌是一种食源性革兰氏阳性病原体，它利用鞭毛提高上皮细胞侵袭的效率（A. Bigot，H。Pagniez，E。Botton，C.Frehel，I。Dubail ，C。Jacquet，A。Charbit和C. Raynaud，Infect。Immun。> 73： 5530-5539，2005; L。Dons，E。Eriksson，Y。Jin，ME Rottenberg，K。 Kristensson，CN Larsen，J.Bresciani，和JE Olsen，Infect.Immun。> 72： 3237-3244，2004）。在这项研究中，我们旨在阐明鞭毛对 L起作用的机制。单核细胞增生病入侵。为了检查鞭毛作为粘附素的作用，对鞭毛运动和非运动细菌以及非鞭毛细菌进行了侵袭和粘附测定。我们观察到，有鞭毛但不运动的细菌比没有鞭毛的细菌不能更有效地粘附或侵袭人类上皮细胞。这些结果表明鞭毛不作为粘附素来增强 L的粘附。单核细胞增生因子靶向宿主细胞相反，看来运动性对于组织培养物的入侵很重要。此外，我们使用竞争指数分析测试了运动性是否有助于胃肠道的早期定居，在竞争性分析中，小鼠被运动和非运动细菌以1：1的比例口服感染。差异细菌计数表明，在感染后的早期时间点，运动菌在肠道菌落中胜过非运动菌。这种差异也反映在12小时后的肝脏浸润中，这表明鞭毛介导的运动力增强了 L。体内细菌摄入后不久就具有单核细胞增生性感染性。

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