• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation >MGA2 or SPT23 Is Required for Transcription of the Δ9 Fatty Acid Desaturase Gene, OLE1, and Nuclear Membrane Integrity in Saccharomyces cerevisiae
【24h】

MGA2 or SPT23 Is Required for Transcription of the Δ9 Fatty Acid Desaturase Gene, OLE1, and Nuclear Membrane Integrity in Saccharomyces cerevisiae

机译：酿酒酵母中Δ9脂肪酸去饱和酶基因OLE1和核膜完整性的转录需要MGA2或SPT23。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

MGA2 and SPT23 are functionally and genetically redundant homologs in Saccharomyces cerevisiae. Both genes are implicated in the transcription of a subset of genes, including Ty retrotransposons and Tyinduced mutations. Neither gene is essential for growth, but mga2 spt23 double mutants are inviable. We have isolated a gene-specific activator, SWI5 , and the Δ9 fatty acid desaturase of yeast, OLE1 , as multicopy suppressors of an mga2 Δ spt23 temperature-sensitive mutation ( spt23-ts ). The level of unsaturated fatty acids decreases 35–40% when the mga2 Δ spt23-ts mutant is incubated at 37°. Electron microscopy of these cells reveals a separation of inner and outer nuclear membranes that is sometimes accompanied by vesicle-like projections in the intermembrane space. The products of Ole1p catalysis, oleic acid and palmitoleic acid, suppress mga2 Δ spt23-ts and mga2 Δ spt23 Δ lethality and restore normal nuclear membrane morphology. Furthermore, the level of the OLE1 transcript decreases more than 15-fold in the absence of wild-type Mga2p and Spt23p. Our results suggest that Mga2p/Spt23p control cell viability by stimulating OLE1 transcription.
机译：MGA2和SPT23是啤酒酵母中功能上和基因上的冗余同源物。这两个基因都与一个基因子集的转录有关，包括Ty逆转座子和Ty诱导的突变。这两个基因都不是生长所必需的，但是mga2 spt23双突变体是不可行的。我们已经分离出基因特异性激活因子SWI5和酵母OLE1的Δ9脂肪酸去饱和酶，作为mga2Δspt23温度敏感突变（spt23-ts）的多拷贝抑制剂。当mga2Δspt23-ts突变体在37°孵育时，不饱和脂肪酸的含量降低35-40％。这些细胞的电子显微镜检查揭示了内核膜和外核膜的分离，有时在膜间空间伴有小泡状突起。 Ole1p催化产物，油酸和棕榈油酸可抑制mga2Δspt23-ts和mga2Δspt23Δ致死率并恢复正常的核膜形态。此外，在没有野生型Mga2p和Spt23p的情况下，OLE1转录物的水平降低了15倍以上。我们的结果表明，Mga2p / Spt23p通过刺激OLE1转录来控制细胞活力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号