...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Interferon induction of gene transcription analyzed by in vivo footprinting.
【24h】

Interferon induction of gene transcription analyzed by in vivo footprinting.

机译：通过体内足迹分析干扰素诱导的基因转录。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The promoters of two interferon-induced genes (the ISG54 and guanylate-binding protein [GBP] genes) have been analyzed in whole cells and in isolated nuclei by using a new genomic sequencing technique. The ISG54 gene contains an interferon-simulating response element (ISRE), earlier shown to be necessary and sufficient for alpha interferon (IFN-alpha) induction, that appeared complexed with proteins in both transcribing and nontranscribing cells. However, the extent of protection and hypersensitivity to DNase I or dimethyl sulfate within the ISRE region was changed upon transcriptional induction, suggesting the binding of different factors in different transcriptional states. In addition to the ISRE, the GBP gene needs a newly recognized DNA element, called the GAS, that partly overlaps the ISRE for full induction by either IFN-alpha or IFN-gamma. This GAS element was transiently protected against DNase I in the nuclei of interferon-treated cells but was not protected at later times when transcription reached maximal levels. Thus, the GAS-binding activity may be necessary only transiently for the initial assembly of a transcription initiation complex on the GBP promoter. Dimethyl sulfate methylation of genomic DNA performed on intact cells showed a characteristic sensitivity over the GAS that correlated with transcription levels and that persisted longer than did DNase I protection over the GAS. These results demonstrate the involvement of the GAS in IFN-alpha and -gamma induction of GBP and suggest the presence of an altered DNA conformation or a small protein in the major groove of the GAS associated with ongoing GBP transcription.
机译：通过使用新的基因组测序技术，已经在全细胞和分离的细胞核中分析了两种干扰素诱导的基因（ISG54和鸟苷酸结合蛋白[GBP]基因）的启动子。 ISG54基因包含干扰素模拟反应元件（ISRE），之前已证明它对诱导α-干扰素（IFN-α）来说是必需和充分的，在转录和非转录细胞中均与蛋白质复合。但是，ISRE区域内对DNase I或硫酸二甲酯的超敏反应的保护程度在转录诱导后发生了变化，表明不同因子在不同转录状态下的结合。除ISRE之外，GBP基因还需要一个新的识别为GAS的DNA元件，该元件与ISRE部分重叠，以被IFN-α或IFN-γ完全诱导。在干扰素处理过的细胞核中，该GAS元件被瞬时保护免受DNase I的侵害，但在后来转录达到最大水平时，则不受保护。因此，仅对于GBP启动子上的转录起始复合物的初始组装而言，GAS结合活性仅是短暂需要的。在完整细胞上进行的基因组DNA的硫酸二甲酯甲基化显示，其对GAS的特征敏感性与转录水平相关，并且比DNase I对GAS的保护持续的时间更长。这些结果证明了GAS参与了GBP的IFN-α和-γ诱导，并暗示了与正在进行的GBP转录相关的GAS的主要沟中DNA构象的改变或小蛋白的存在。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号