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首页> 外文期刊>RSC Advances >Double-ratiometric fluorescence imaging of H2Se and O2˙? under hypoxia for exploring Na2SeO3-induced HepG2 cells' apoptosis
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Double-ratiometric fluorescence imaging of H2Se and O2˙? under hypoxia for exploring Na2SeO3-induced HepG2 cells' apoptosis

机译：H2Se和O2˙的双比例荧光成像在缺氧条件下探索Na2SeO3诱导的HepG2细胞凋亡

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Sodium selenite (Na _(2) SeO _(3) ), as an anti-tumor drug for inducing tumor cells' apoptosis, has been widely studied under normoxic conditions and has been shown to exhibit oxidative stress process-induced apoptosis. However, since the real tumor environment is hypoxic, the actual mechanism is still unclear. Hence, considering the main metabolite of Na _(2) SeO _(3) in the metabolic process to be hydrogen selenide (H _(2) Se) and also that it can be converted to superoxide anion (O _(2) ˙ ~(?) ) instantaneously in the presence of oxygen, a dual-ratiometric fluorescence imaging system for simultaneous monitoring of the changes of H _(2) Se and O _(2) ˙ ~(?) induced by Na _(2) SeO _(3) -guided tumor cell apoptosis under hypoxic conditions was constructed. Two molecular probes NIR-H _(2) Se and dihydroethidium were used to detect H _(2) Se and O _(2) ˙ ~(?) , respectively, whereas Rhodamine 110 was used as the reference fluorophore. Notably, H _(2) Se levels significantly increased under hypoxic conditions, but there was no change in the level of O _(2) ˙ ~(?) , which is inconsistent with the results of the previous researches. Therefore, we hypothesize that the mechanism of Na _(2) SeO _(3) -induced apoptosis for tumor cells is caused by reductive stress; also, this method can be applied for the future study of other anti-cancer selenium compounds.
机译：亚硒酸钠（Na _（2）SeO _（3））作为诱导肿瘤细胞凋亡的抗肿瘤药物，已在常氧条件下得到了广泛研究，并已显示出氧化应激过程诱导的细胞凋亡。但是，由于实际的肿瘤环境是缺氧的，其实际机制仍不清楚。因此，考虑到代谢过程中Na _（2）SeO _（3）的主要代谢物是硒化氢（H _（2）Se），而且它还可以转化为超氧阴离子（O _（2）˙ 〜（？））在有氧的情况下瞬间完成，一个双比率荧光成像系统可同时监测Na _（2）诱导的H _（2）Se和O _（2）˙〜（？）的变化。在缺氧条件下构建了SeO_（3）指导的肿瘤细胞凋亡。使用两个分子探针NIR-H _（2）Se和二氢乙啶分别检测H _（2）Se和O _（2）˙〜（η），而若丹明110用作参考荧光团。值得注意的是，在缺氧条件下H _（2）Se含量显着增加，但是O _（2）˙〜（？）的水平没有变化，这与先前的研究结果不一致。因此，我们假设Na _（2）SeO _（3）诱导肿瘤细胞凋亡的机制是由还原性应激引起的。而且，该方法可用于将来其他抗癌硒化合物的研究。

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