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首页> 外文期刊>RSC Advances >An innovative biotransformation to produce resveratrol by Bacillus safensis
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An innovative biotransformation to produce resveratrol by Bacillus safensis

机译：安全芽孢杆菌产生白藜芦醇的创新生物转化

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Resveratrol is considered as a potential food supplement, cosmetic ingredient and nutraceutical. In this study, resveratrol was produced by biotransformation successfully. In detail, a β-glucosidase producing strain was isolated and identified as Bacillus safensis , and it could convert polydatin to resveratrol efficiently and rapidly. Further research showed that the conversion rate to resveratrol reached 93.1% in 8 h at 37 °C. The production of resveratrol was confirmed by HPLC, LC-MS and ~(1) H-NMR to identify its structure and it was verified to possess antibacterial properties especially against Escherchia coli. To illustrate the resveratrol transformation mechanism, several glucosidases from B. safensis CGMCC 13129 were expressed and analyzed. The results showed that BGL4 and BGL5 had higher transformation activity compared with other tested glucosidases. This research provides a novel approach to produce resveratrol, and would promote the application of resveratrol in health-promoting pharmaceutical and food products.
机译：白藜芦醇被认为是潜在的食品补充剂，化妆品成分和营养保健品。在这项研究中，成功​​地通过生物转化生产了白藜芦醇。详细地，分离出了产生β-葡糖苷酶的菌株，并将其鉴定为安全芽孢杆菌，其可以有效地和快速地将多角蛋白转化为白藜芦醇。进一步的研究表明，在37°C下8 h，白藜芦醇的转化率达到93.1％。通过HPLC，LC-MS和〜（1）H-NMR确认白藜芦醇的产生以鉴定其结构，并证实其具有抗菌性能，尤其是对大肠杆菌的抗菌性能。为了说明白藜芦醇转化机制，表达并分析了来自安全双歧杆菌CGMCC 13129的几种葡糖苷酶。结果表明，与其他测试的葡糖苷酶相比，BGL4和BGL5具有更高的转化活性。这项研究提供了一种生产白藜芦醇的新方法，并将促进白藜芦醇在促进健康的药品和食品中的应用。

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