Identification of OmpR: a positive regulatory protein controlling expression of the major outer membrane matrix porin proteins of Escherichia coli K-12.

R K Taylor; M N Hall; L Enquist; T J Silhavy

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Identification of OmpR: a positive regulatory protein controlling expression of the major outer membrane matrix porin proteins of Escherichia coli K-12.

机译：OmpR的鉴定：一种阳性调节蛋白，可控制大肠杆菌K-12主要外膜基质孔蛋白的表达。

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We report here on the cloning of a gene located within the ompB locus of E. coli K-12. This gene, designated ompR, resides on a 10.9-kilobase EcoRI fragment that we cloned, using a lambda vector and in vitro packaging techniques. By subcloning portions of this fragment into the high-copy-number plasmid pBR322, we have isolated the ompR gene on a 1.4-kilobase EcoRI-AvaI fragment. This fragment has been characterized physically and will facilitate a more detailed study of the role and mechanism of porin regulation by the ompB locus.

机译：我们在这里报告了位于大肠杆菌K-12的ompB位点内的基因的克隆。使用lambda载体和体外包装技术，该基因称为ompR，位于我们克隆的10.9千碱基EcoRI片段上。通过将该片段的部分亚克隆到高拷贝数质粒pBR322中，我们已经在1.4碱基碱基的EcoRI-AvaI片段上分离了ompR基因。该片段已被物理表征，将有助于对ompB基因座调控孔蛋白的作用和机制进行更详细的研究。

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《Journal of bacteriology》 |1981年第1期|共4页
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R K Taylor; M N Hall; L Enquist; T J Silhavy;
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1. Identification of three genes controlling production of new outer membrane pore proteins in Escherichia coli K-12. [J] . A P Pugsley, C A Schnaitman Journal of bacteriology . 1978,第3期

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2. Environmental Regulation of Campylobacter jejuni Major Outer Membrane Protein Porin Expression in Escherichia coli Monitored by Using Green Fluorescent Protein [J] . Luc Dedieu, Jean-Marie Pagès, Jean-Michel Bolla Applied and Environmental Microbiology . 2002,第9期

机译：使用绿色荧光蛋白监测空肠弯曲菌主要外膜蛋白孔蛋白在大肠杆菌中的环境调控
3. Specialized transducing bacteriophage lambda carrying the structural gene for a major outer membrane matrix protein of Escherichia coli K-12. [J] . N Mutoh, T Nagasawa, S Mizushima Journal of bacteriology . 1981,第2期

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4. Effect of silver coated carbon nanotubes on metabolically essential genes expression and outer membrane protein profile of Escherichia coli [C] . Aral A Chaudhari, Shree R. Singh, Shreekumar Pillai Biotech, biomaterials and biomedical . 2016

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5. Expression of the Chlamydia psittaci major outer membrane protein in Escherichia coli: A model for translocation and vaccine development. [D] . Dascher, Christopher Carter. 1994

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6. Identification of OmpR: a positive regulatory protein controlling expression of the major outer membrane matrix porin proteins of Escherichia coli K-12. [O] . R K Taylor, M N Hall, L Enquist, 1981

机译：OmpR的鉴定：一种正调控蛋白可控制大肠杆菌K-12主要外膜基质孔蛋白的表达。
7. Identification of OmpR: a positive regulatory protein controlling expression of the major outer membrane matrix porin proteins of Escherichia coli K-12 [O] . R K Taylor, M N Hall, L Enquist, 1981

机译：综合综合综合征：大肠杆菌K-12主要外膜基质诱导蛋白的阳性调节蛋白质控制表达
8. Expression in Escherichia coli K-12 of the 76, 000-Dalton Iron-Regulated Outer Membrane Protein of Shigella flexneri Confers Sensitivity to Cloacin DF13 in the Absence of Shigella O Antigen. (Reannouncement with New Availability Information). [R] . Derbyshire, U., Baldwin, T., Stevenson, P., 1989

机译：在福氏志贺菌的76,000道尔顿铁调节的外膜蛋白的大肠杆菌K-12中的表达赋予在没有志贺氏菌抗原的情况下对Cloacin DF13的敏感性。（重新公布新的可用性信息）。

Identification of OmpR: a positive regulatory protein controlling expression of the major outer membrane matrix porin proteins of Escherichia coli K-12.

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