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首页> 外文期刊>Journal of bacteriology >The REP2 Repeats of the Genome of Neisseria meningitidis Are Associated with Genes Coordinately Regulated during Bacterial Cell Interaction
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The REP2 Repeats of the Genome of Neisseria meningitidis Are Associated with Genes Coordinately Regulated during Bacterial Cell Interaction

机译：脑膜炎奈瑟氏球菌基因组的REP2重复与细菌细胞相互作用期间协调调节的基因有关。

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Interaction with host cells is essential in meningococcal pathogenesis especially at the blood-brain barrier. This step is likely to involve a common regulatory pathway allowing coordinate regulation of genes necessary for the interaction with endothelial cells. The analysis of the genomic sequence of Neisseria meningitidis Z2491 revealed the presence of many repeats. One of these, designated REP2, contains a ?24/?12 type promoter and a ribosome binding site 5 to 13 bp before an ATG. In addition most of these REP2 sequences are located immediately upstream of an ORF. Among these REP2-associated genes are pilC1 and crgA, described as being involved in steps essential for the interaction of N. meningitidis with host cells. Furthermore, the REP2 sequences located upstream of pilC1 and crgA correspond to the previously identified promoters known to be induced during the initial localized adhesion of N. meningitidis with human cells. This characteristic led us to hypothesize that at least some of the REP2-associated genes were upregulated under the same circumstances as pilC1 and crgA. Quantitative PCR in real time demonstrated that the expression of 14 out of 16 REP2-associated genes were upregulated during the initial localized adhesion of N. meningitidis. Taken together, these data suggest that these repeats control a set of genes necessary for the efficient interaction of this pathogen with host cells. Subsequent mutational analysis was performed to address the role of these genes during meningococcus-cell interaction.
机译：与宿主细胞的相互作用在脑膜炎球菌的发病机理中至关重要，尤其是在血脑屏障中。该步骤可能涉及共同的调节途径，该途径允许协调调节与内皮细胞相互作用所必需的基因。对脑膜炎奈瑟氏球菌Z2491的基因组序列的分析表明存在许多重复序列。这些中的一个，称为REP2，在ATG之前含有α24/β12型启动子和核糖体结合位点5至13bp。另外，大多数这些REP2序列位于ORF的紧邻上游。在这些与REP2相关的基因中，有 pilC1 和 crgA ，它们被描述为与 N相互作用必不可少的步骤。脑膜炎与宿主细胞。此外，位于 pilC1 和 crgA 上游的REP2序列对应于先前确定的已知启动子，这些启动子已知是在 N的初始局部粘附过程中诱导的。脑膜炎与人类细胞。这一特征使我们假设，在与 pilC1 和 crgA 相同的情况下，至少一些与REP2相关的基因被上调。实时定量PCR证实，在 N的最初局部黏附过程中，与REP2相关的16个基因中有14个的表达上调。脑膜炎。综上所述，这些数据表明这些重复序列控制了该病原体与宿主细胞有效相互作用所必需的一组基因。随后进行了突变分析，以解决这些基因在脑膜炎双球菌细胞相互作用中的作用。

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