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首页> 外文期刊>Cancer Cell International >Anti-cancer effect of gallic acid in presence of low level laser irradiation: ROS production and induction of apoptosis and ferroptosis
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Anti-cancer effect of gallic acid in presence of low level laser irradiation: ROS production and induction of apoptosis and ferroptosis

机译：在低水平激光照射存在下的抗癌作用：ROS生产和诱导细胞凋亡和脱盐

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摘要

There are different treatments for breast cancer and melanoma that mostly have some side effects. One of the therapeutic strategies is the use of natural components. Phenol components are a class of antioxidants in plants that have many biological functions like anticancer effects. Gallic acid (GA) is a natural polyhydroxy phenolic compound and commonly found in various foods. In the present study, GA effects alone and in combination with low-level laser irradiation on human dermal fibroblast cell line (HDF), human non-tumorigenic breast epithelial cell line (MCF10A), breast cancer cell line (MDA-MB-231) and melanoma cancer cell line (A375) was under the investigation. The normal and cancerous cell lines were exposed to 660?nm low-level laser with 3?J/cm2 for 90?s. Then, the cells were treated with different concentrations of GA for 24?h. In another study, the cell lines firstly were treated with GA and then exposed to low-level laser irradiation. The effects of GA and low-level laser on cell survival and apoptosis were examined using MTT assay, light microscopy, ROS production assay, fluorescence microscopy (AO/EB double staining) and flow cytometry. The results showed that pre-treatment with low-level laser and then GA reduced the survival of breast cancer cells and melanoma more than the first treatment with GA and then low-level laser irradiation. Our findings showed that ROS production in cells treated with both low-level laser and GA was more than the cells treated with GA alone. The apoptosis and ferroptosis assays confirmed the MTT results which combination treatment with low-level laser and then GA increase the cell death probably via apoptosis and ferroptosis cell death mechanisms compared to GA alone. This study suggests that low-level laser irradiation alone is not able to cause death in human normal and cancerous cells. Preirradiation followed by GA treatment did not change the cell viability in human normal significantly but reduces the cell survival of cancer cells more than GA alone.
机译：对乳腺癌和黑色素瘤有不同的治疗方法，大多数有一些副作用。其中一种治疗策略是使用天然成分。苯酚组分是一类植物中的抗氧化剂，具有许多生物学功能，如抗癌效果。加仑酸（Ga）是天然多羟基酚类化合物，常见于各种食物中。在本研究中，对人类皮肤成纤维细胞系（HDF），人非致瘤乳腺上皮细胞系（MCF10A），乳腺癌细胞系（MDA-MB-231）（MDA-MB-231）（MDA-MB-231）（MDA-MB-231）而单独使用GA效果。和黑色素瘤癌细胞系（A375）在调查中。将正常和癌细胞系暴露于660℃的低水平激光器，3. j / cm2持续90. s。然后，用不同浓度的Ga处理细胞24μm。在另一个研究中，首先用Ga处理细胞系，然后暴露于低水平的激光照射。使用MTT测定，光学显微镜，ROS产生测定，荧光显微镜（AO / EB双染色）和流式细胞术检查Ga和低水平激光对细胞存活和细胞凋亡的影响。结果表明，用低水平激光进行预处理，然后将乳腺癌细胞和黑色素瘤的存活减少，比使用Ga，然后是低水平的激光照射的第一次处理。我们的研究结果表明，用低水平激光和GA处理的细胞中的ROS产生比单独使用GA处理的细胞。细胞凋亡和脱盐菌测定证实了MTT结果，其用低水平激光器组合治疗，然后通过单独使用细胞凋亡和脱盐细胞死亡机制增加细胞死亡。本研究表明，单独的低水平激光照射不能导致人类正常和癌细胞死亡。 Prearadiation，然后进行Ga治疗并未显着改变人体中的细胞活力，但仅减少癌细胞的细胞存活而不是单独的。

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