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Kinesin-5 Eg5 is essential for spindle assembly and chromosome alignment of mouse spermatocytes

机译：Kinesin-5 eg5对于小鼠精子组件和染色体对准是必不可少的小鼠精子胶质细胞

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Microtubule organization is essential for bipolar spindle assembly and chromosome segregation, which contribute to genome stability. Kinesin-5 Eg5 is known to be a crucial regulator in centrosome separation and spindle assembly in mammalian somatic cells, however, the functions and mechanisms of Eg5 in male meiotic cell division remain largely unknown. In this study, we have found that Eg5 proteins are expressed in mouse spermatogonia, spermatocytes and spermatids. After Eg5 inhibition by specific inhibitors Monastrol, STLC and Dimethylenastron, the meiotic spindles of dividing spermatocytes show spindle collapse and the defects in bipolar spindle formation. We demonstrate that Eg5 regulates spindle bipolarity and the maintenance of meiotic spindles in meiosis. Eg5 inhibition leads to monopolar spindles, spindle abnormalities and chromosome misalignment in cultured GC-2 spd cells. Furthermore, Eg5 inhibition results in the decrease of the spermatids and the abnormalities in mature sperms. Our results have revealed an important role of kinesin-5 Eg5 in male meiosis and the maintenance of male fertility. We demonstrate that Eg5 is crucial for bipolar spindle assembly and chromosome alignment in dividing spermatocytes. Our data provide insights into the functions of Eg5 in meiotic spindle assembly of dividing spermatocytes.
机译：微管组织对于双极主轴组件和染色体偏析至关重要，这有助于基因组稳定性。众所周知，Kinesin-5 eG5是在哺乳动物体细胞中的中心分离和主轴组件中的关键调节剂，然而，EG5在雄性减肥细胞分裂中的功能和机制仍然很大程度上是未知的。在这项研究中，我们发现EG5蛋白质在小鼠精子，精子细胞和精菌中表达。在EG5抑制特异性抑制剂Monastrol，STLC和二甲基亚替代之后，分裂精子细胞的减数分裂纺织纺锤体塌陷和双极纺锤体形成中的缺陷。我们证明EG5调节主轴两极和减数分裂中减数分裂的维持。 EG5抑制导致培养的GC-2 SPD细胞中的单极纺锤体，主轴异常和染色体未对准。此外，EG5抑制导致精子的降低和成熟精子的异常。我们的结果揭示了Kinesin-5 Eg5在雄性分裂中的重要作用以及对男性生育的维持。我们证明EG5对于双极主轴组件和分割精子细胞中的染色体对准是至关重要的。我们的数据提供了对分割精子细胞的减数分子主轴组件的EG5功能的见解。

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