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Competition between Granule Bound Starch Synthase and Starch Branching Enzyme in Starch Biosynthesis

机译：淀粉生物合成中颗粒结合淀粉合酶与淀粉分支酶的竞争

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Abstract BackgroundStarch branching enzymes (SBE) and granule-bound starch synthase (GBSS) are two important enzymes for starch biosynthesis. SBE mainly contributes to the formation of side branches, and GBSS mainly contributes for the synthesis of amylose molecules. However, there are still gaps in the understanding of possible interactions between SBE and GBSS.ResultsNineteen natural rice varieties with amylose contents up to 28% were used. The molecular structure, in the form of the chain-length distribution (CLDs, the distribution of the number of monomer units in each branch) was measured after enzymatic debranching, using fluorophore-assisted carbohydrate electrophoresis for amylopectin and size- exclusion chromatography for amylose. The resulting distributions were fitted to two mathematical models based on the underlying biosynthetic processes, which express the CLDs in terms of parameters reflecting relevant enzyme activities.ConclusionsFinding statistically valid correlations between the values of these parameters showed that GBSSI and SBEI compete for substrates during rice starch biosynthesis, and synthesis of amylose short chains involves several enzymes including GBSSI, SBE and SSS (soluble starch synthase). Since the amylose CLD is important for a number of functional properties such as digestion rate, this knowledge is potentially useful for developing varieties with improved functional properties.
机译：摘要背景架构支化酶（SBE）和颗粒结合的淀粉合酶（GBS）是淀粉生物合成的两个重要酶。 SBE主要有助于形成侧支分支，GBSS主要有助于合成直链淀粉分子。然而，在理解SBE和GBSS的可能相互作用仍然存在差距。使用含有直链淀粉含量的天然水稻品种高达28％。以链长分布（CLD的形式）的分子结构在酶促脱胆后测量酶促脱蛋白的碳化素辅助碳水化合物和直链淀粉的尺寸排阻色谱法测定酶促脱氮后的形式的形式。得到的分布适用于基于底层的生物合成过程的两种数学模型，其在反映相关酶活性的参数方面表达CLD。结合这些参数的值之间的统计有效相关性，显示GBSSI和Sbei在水稻淀粉期间竞争底物。生物合成和淀粉短链的合成涉及几种酶，包括GBSSI，SBE和SSS（可溶性淀粉合酶）。由于淀粉糖CLD对于许多功能性（例如消化率）重要，因此该知识可能对开发具有改善功能性质的品种有用。

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