Crosslinking catalysis-active center of hemin on the protein scaffold toward peroxidase mimic with powerful catalysis

Minmin Dong; Liyan Zhang; Rui Li; Shuying Li; Yao Jiang; Yuchun Qiao; Zhiqiang Duan; Ru Li; Quanfu Wang; Hua Wang

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Crosslinking catalysis-active center of hemin on the protein scaffold toward peroxidase mimic with powerful catalysis

机译：用强大的催化掺入过氧化物酶的蛋白质支架上的血红素催化激活中心

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An enzyme mimic synthesis protocol has been proposed by simply cross-linking the redox active center of peroxidase onto a protein scaffold. Colorimetric assays and kinetic studies indicate that the developed peroxidase mimic can present much stronger catalysis and better aqueous stability than native hemin.

机译：通过简单地将过氧化物酶的氧化还原活性酶交联在蛋白质支架上提出了一种酶模拟合成方案。比色测定和动力学研究表明，发育的过氧化物酶模拟物可以具有比天然血红素更强的催化和更好的含水稳定性。

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《RSC Advances》 |2016年第53期|共5页
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Minmin Dong; Liyan Zhang; Rui Li; Shuying Li; Yao Jiang; Yuchun Qiao; Zhiqiang Duan; Ru Li; Quanfu Wang; Hua Wang;
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1. Crosslinking catalysis-active center of hemin on the protein scaffold toward peroxidase mimic with powerful catalysis [J] . Dong Minmin, Zhang Liyan, Li Rui, RSC Advances . 2016,第53期

机译：用强大的催化掺入过氧化物酶的蛋白质支架上的血红素催化激活中心
2. Cu-hemin metal-organic frameworks with peroxidase-like activity as peroxidase mimics for colorimetric sensing of glucose [J] . Liu Fenfen, He Juan, Zeng Mulang, Journal of nanoparticle research: An interdisciplinary forum for nanoscale science and technology . 2016,第5期

机译：具有过氧化物酶样活性的铜-血红素金属有机骨架，作为过氧化物酶模拟物，用于比色检测葡萄糖
3. Biominerized gold-Hemin@MOF composites with peroxidase-like and gold catalysis activities: A high-throughput colorimetric immunoassay for alpha-fetoprotein in blood by ELISA and gold-catalytic silver staining [J] . Liyan Zhang, Chuan Fan, Min Liu, Sensors and Actuators . 2018,第auga期

机译：生物矿化的具有过氧化物酶样和金催化活性的金Hemin @ MOF复合物：通过ELISA和金催化银染的高通量比色免疫法测定血液中甲胎蛋白
4. Breast cancer diagnosis system using the combination of carcinoembryonic antigen aptamer, horseradish peroxidase-mimic hemin, and graphene sheet [C] . Stephanie Chong, Kelly Cho, Harriet J. Khang, National SBIR/STTR conference;Annual nanotech conference and expo;Annual TechConnect world innovation conference expo . 2015

机译：结合癌胚抗原适体，辣根过氧化物酶模拟血红素和石墨烯片的乳腺癌诊断系统
5. Engineering protein scaffolds for protein separation, biocatalysis and nanotechnology applications [D] . Liu, Fang 2013

机译：工程蛋白质支架，用于蛋白质分离，生物催化和纳米技术应用
6. Crosslinking of hemin to a specific site on the 90-kDa ferritin repressor protein. [O] . J J Lin, M M Patino, L Gaffield, 1991

机译：血红素交联到90 kDa铁蛋白阻遏蛋白上的特定位点。
7. G-quadruplex based impedimetric 2-hydroxyfluorene biosensor using hemin as a peroxidase enzyme mimic [O] . Liang Gang, Liu Xinhui 2015

机译：基于G四联体的阻抗式2-羟基芴生物传感器，使用血红素作为过氧化物酶模拟酶

Crosslinking catalysis-active center of hemin on the protein scaffold toward peroxidase mimic with powerful catalysis

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