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首页> 外文期刊>Endocrinology, Diabetes & Metabolism >Glioblastoma cells express crucial enzymes involved in androgen synthesis: 3β-hydroxysteroid dehydrogenase, 17-20α-hydroxylase, 17β-hydroxysteroid dehydrogenase and 5α-reductase
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Glioblastoma cells express crucial enzymes involved in androgen synthesis: 3β-hydroxysteroid dehydrogenase, 17-20α-hydroxylase, 17β-hydroxysteroid dehydrogenase and 5α-reductase

机译：胶质细胞瘤细胞表达参与雄激素合成的关键酶：3β-羟类脱氢酶，17-20α-羟化酶，17β-羟类脱氢酶和5α-还原酶

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Glioblastoma (GB) is the most?common?and aggressive primary brain tumour in adult humans. Therapeutic resistance and tumour recurrence after surgical removal?contribute to?poor prognosis?for glioblastoma patients.?Men are known to be more likely than women to develop an aggressive form of GB, and differences in sex steroids have emerged as a leading explanation?for this finding. Studies indicate that?the metabolism and proliferation of?GB-derived cells?are increased?by sex steroids,?the?expression of?androgen receptors (ARs)?and?the?synthesis of?androgens?and oestrogens, suggesting that?these hormones?have a role in the tumour pathogenesis.?The expression of aromatase,?the?enzyme that converts androgens to oestrogens, has?been?reported in glial cells and GB?cell lines. Thus, it?was?necessary?to?test whether?the steroidogenic enzymes involved in androgen synthesis are expressed in GB cells.?Therefore,?here,?we?investigated the expression of four?key enzymes involved in androgen synthesis in human-derived GB?cells.?U87 cells were cultured in?Dulbecco's?modified Eagle?medium?plus?foetal bovine serum?and antibiotics on slides for immunocytochemistry or immunofluorescence. U87, LN229 and C6 cells were also cultured in multi-well?chambers?to obtain proteins for Western?blotting.?We?used?primary antibodies against?3β-hydroxysteroid dehydrogenase (3β-HSD), 17α-hydroxilase/17,20-lyase (P450c17),?17β-hydroxysteroid dehydrogenase (17β-HSD)?and 5α-reductase.?Immunocytochemistry, and?immunofluorescence?results revealed that?glioblastoma?cells?express 3β-HSD, P450c17, 17β-HSD and 5α-reductase?proteins in their cytoplasm. Moreover, Western?blot analyses revealed bands corresponding to the?molecular weight?of these?four enzymes in the three GB cell lines.?Thus, glioblastoma cells have?the key enzymatic machinery necessary to synthesize androgens,?and these enzymes?might?be?useful?targets?for new?therapeutic?approaches.
机译：胶质母细胞瘤（GB）是最常见的？和成年人中的侵略性原发性脑肿瘤。手术后患者的治疗性和肿瘤复发？有助于？预后差？对于胶质母细胞瘤患者。众所周知，有可能比妇女更有可能发展侵略性的GB形式，而性类固醇的差异被出现为领先的解释？这一发现。研究表明？GB衍生细胞的新陈代谢和增殖？通过性类固醇增加？？？α和α雌激素受体（ARS）的表达？和？合成？和雌激素，暗示？这些激素？在肿瘤发病机制中有一种作用。芳香酶的表达，α，转化对雌激素的酶，已经？在胶质细胞和GB？细胞中报道。因此？是？必要的？对象是吗？是否在GB细胞中表达雄激素合成中所涉及的类固化酶。？原因,? -One，[Where，[Where]的表达，在人类中参与雄激素合成的关键酶的表达衍生的gb？细胞。 U87，LN229和C6细胞也培养在多孔孔中？腔室？为西方蛋白质获得蛋白质？出版物。？我们？使用？用α-3β-羟类脱氢酶（3β-HSD），17α-羟基胺/ 17,20 -Lyase（p450c17），17β-羟类脱氢酶（17β-HSD）？和5α-还原酶。？免疫细胞化学，和α免疫荧光α。结果显示？胶质母细胞瘤？细胞？表达3β-HSD，P450C17，17β-HSD和5α-还原酶？它们的细胞质中的蛋白质。此外，Western？污点分析揭示了与α分子量相对应的条带？其中的三种GB细胞系中的四种酶。？胶质母细胞瘤细胞具有α，伴有雄性糖酵发所需的关键酶促机械，？和这些酶？是什么？有用吗？目标？对于新的？治疗？方法。

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