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首页> 外文期刊>Environmental research >Bacterial response mechanism during biofilm growth on different metal material substrates: EPS characteristics, oxidative stress and molecular regulatory network analysis
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Bacterial response mechanism during biofilm growth on different metal material substrates: EPS characteristics, oxidative stress and molecular regulatory network analysis

机译：不同金属材料底物生物膜生长过程中的细菌反应机制：EPS特征，氧化应激和分子调节网络分析

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摘要

Overwhelming growth of bacterial biofilms on different metal-based pipeline materials are intractable and pose a serious threat to public health when tap water flows though these pipelines. Indeed, the underlying mechanism of biofilm growth on the surface of different pipeline materials deserves detailed exploration to provide subsequent implementation strategies for biofilm control. Thus, in this study, how bacteria response to their encounters was explored, when they inhabit different metal-based pipeline substrates. Results revealed that bacteria proliferated when they grew on stainless steel (SS) and titanium sheet (Ti), quickly developing into bacterial biofilms. In contrast, the abundance of bacteria on copper (Cu) and nickel foam (Ni) substates decreased sharply by 4-5 logs within 24 h. The morphological shrinkage and shortening of bacterial cells, as well as a sudden 64-fold increase of carbohydrate content in extracellular polymeric substances (EPS), were observed on Cu substrate. Furthermore, generation of reactive oxygen species and fluctuation of enzymatic activity demonstrated the destruction of redox equilibrium in bacteria. Bacteria cultured on Cu substrate showed the strongest response, followed by Ni, SS and Ti. The oxidative stress increased quickly during the growth of bacterial biofilm, and almost all tested metal transporter-related genes were upregulated by 2-11 folds on Cu, which were higher than on other substrates (1-2 folds for SS and Ti, 2-9 folds for Ni). Finally, these behaviors were compared under the biofilm regulatory molecular network. This work may facilitate better understanding different response mechanisms during bacterial biofilm colonization on metal-based pipelines and provide implications for subsequent biofilm control.
机译：在不同金属的管道材料上的细菌生物膜的压倒性生长是棘手的，并且当自来水虽然这些管道流动时，对公共健康产生严重威胁。实际上，生物膜生长在不同管道材料表面上的潜在机制值得详细探索，以提供后续实施策略对生物膜控制。因此，在本研究中，当他们居住不同的基于金属的管道基板时，探索了如何对其遭遇的反应。结果表明，当它们在不锈钢（SS）和钛板（TI）上延长时，细菌增殖，迅速发展成细菌生物膜。相反，铜（Cu）和镍泡沫（Ni）变化物上的细菌的丰度在24小时内急剧下降4-5个原木。在Cu底物上观察到细胞细胞的形态收缩和缩短细胞细胞中碳水化合物含量的突然64倍，碳水化合物含量增加。此外，对反应性氧物质的产生和酶活性的波动证明了细菌中氧化还原平衡的破坏。在Cu底物上培养的细菌显示出最强的反应，其次是Ni，SS和Ti。在细菌生物膜的生长过程中氧化应激迅速增加，并且几乎所有测试的金属转运蛋白相关基因在Cu上升高了2-11倍，其高于其他基材（SS和Ti折叠，2- 9倍为ni）。最后，在生物膜调节分子网络下比较了这些行为。这项工作可以促进在基于金属的管道上的细菌生物膜定植过程中更好地理解不同的响应机制，并对随后的生物膜控制提供影响。

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  • 来源
    《Environmental research》 |2020年第6期|109451.1-109451.10|共10页
  • 作者

    Jiaping Wang; Guiying Li; Hongliang Yin; Taicheng An;

  • 作者单位

    Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control Guangzhou Key Laboratory Environmental Catalysis and Pollution Control School of Environmental Science and Engineering Institute of Environmental Health and Pollution Control Guangdong University of Technology Guangzhou 510006 China;

    Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control Guangzhou Key Laboratory Environmental Catalysis and Pollution Control School of Environmental Science and Engineering Institute of Environmental Health and Pollution Control Guangdong University of Technology Guangzhou 510006 China;

    Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control Guangzhou Key Laboratory Environmental Catalysis and Pollution Control School of Environmental Science and Engineering Institute of Environmental Health and Pollution Control Guangdong University of Technology Guangzhou 510006 China;

    Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control Guangzhou Key Laboratory Environmental Catalysis and Pollution Control School of Environmental Science and Engineering Institute of Environmental Health and Pollution Control Guangdong University of Technology Guangzhou 510006 China;

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  • 正文语种 eng
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  • 关键词

    Bacterial biofilm; Growth profiles; EPS; Oxidative stress; Metal-based pipeline materials;

    机译：细菌生物膜;成长型材;EPS;氧化胁迫;金属基管道材料;

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