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首页> 外文期刊>Food Chemistry >Characterization of enzymes specifically producing chiral flavor compounds (R)- and (S)-1-phenylethanol from tea (Camellia sinensis) flowers
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Characterization of enzymes specifically producing chiral flavor compounds (R)- and (S)-1-phenylethanol from tea (Camellia sinensis) flowers

机译：从茶花中特别产生手性风味化合物（R）-和（S）-1-苯基乙醇的酶的表征

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摘要

1-Phenylethanol is a chiral flavor compound that has enantiomers, (R)- and (S)-1-phenylethanol, with different flavor properties. Given that isolating these enantiomers from plants is low yielding and costly, enzymatic synthesis presents an alternative approach. However, the genes/enzymes that specifically produce (R)- and (S)-1-phenylethanol in plants are unknown. To identify these enzymes in tea (Camellia sinensis) flowers, 21 short chain dehydrogenase (SDR) genes were isolated from tea flowers, cloned, and functionally characterized. Several recombinant SDRs in Escherichia coli exhibited activity for converting acetophenone to (S)-1-phenylethanol (CsSPESs, 99.0%), while only one SDR produced (R)-1-phenylethanol (CsRPES, 98.6%). A pair of homologue enzymes (CsSPES and CsRPES) showed a strong preference for NADPH cofactor, with optimal enzymatic reaction conditions of 45-55 degrees C and pH 8.0. Identification of the tea flower-derived gene responsible for specific synthesis of (R)- and (S)-1-phenylethanolsuggests enzymatic synthesis of enantiopure 1-phenylethanol is possible using a plant-derived gene.
机译：1-苯乙醇是一种手性风味化合物，具有对映体（R）-和（S）-1-苯基乙醇，具有不同的风味特性。鉴于从植物中分离这些对映异构体的产率低且成本高，酶促合成提出了另一种方法。但是，尚不清楚在植物中特异性产生（R）-和（S）-1-苯基乙醇的基因/酶。为了鉴定茶（茶树）花中的这些酶，从茶花中分离出21种短链脱氢酶（SDR）基因，进行了克隆和功能鉴定。大肠杆菌中的几种重组SDR表现出将苯乙酮转化为（S）-1-苯基乙醇（CsSPESs，> 99.0％）的活性，而只有一种SDR产生了（R）-1-苯基乙醇（CsRPES，98.6％）。一对同源酶（CsSPES和CsRPES）显示出对NADPH辅因子的强烈偏好，最佳的酶促反应条件是45-55摄氏度和pH 8.0。使用植物来源的基因，可以鉴定负责（R）-和（S）-1-苯基乙醇的特异性合成的茶花来源的基因。建议通过酶法合成对映纯的1-苯基乙醇。

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  • 来源
    《Food Chemistry》 |2019年第may15期|27-33|共7页
  • 作者

    Zhou Ying; Peng Qiyuan; Zhang Ling; Cheng Sihua; Zeng Lanting; Dong Fang; Yang Ziyin;

  • 作者单位

    Chinese Acad Sci, South China Bot Garden, Guangdong Prov Key Lab Appl Bot, Xingke Rd 723, Guangzhou 510650, Guangdong, Peoples R China|Chinese Acad Sci, South China Bot Garden, Key Lab South China Agr Plant Mol Anal & Genet Im, Xingke Rd 723, Guangzhou 510650, Guangdong, Peoples R China|Univ Chinese Acad Sci, Coll Adv Agr Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China;

    Chinese Acad Sci, South China Bot Garden, Guangdong Prov Key Lab Appl Bot, Xingke Rd 723, Guangzhou 510650, Guangdong, Peoples R China|Chinese Acad Sci, South China Bot Garden, Key Lab South China Agr Plant Mol Anal & Genet Im, Xingke Rd 723, Guangzhou 510650, Guangdong, Peoples R China|Univ Chinese Acad Sci, Coll Adv Agr Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China;

    Chinese Acad Sci, South China Bot Garden, Guangdong Prov Key Lab Appl Bot, Xingke Rd 723, Guangzhou 510650, Guangdong, Peoples R China|Chinese Acad Sci, South China Bot Garden, Key Lab South China Agr Plant Mol Anal & Genet Im, Xingke Rd 723, Guangzhou 510650, Guangdong, Peoples R China;

    Chinese Acad Sci, South China Bot Garden, Guangdong Prov Key Lab Appl Bot, Xingke Rd 723, Guangzhou 510650, Guangdong, Peoples R China|Chinese Acad Sci, South China Bot Garden, Key Lab South China Agr Plant Mol Anal & Genet Im, Xingke Rd 723, Guangzhou 510650, Guangdong, Peoples R China|Univ Chinese Acad Sci, Coll Adv Agr Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China;

    Chinese Acad Sci, South China Bot Garden, Guangdong Prov Key Lab Appl Bot, Xingke Rd 723, Guangzhou 510650, Guangdong, Peoples R China|Chinese Acad Sci, South China Bot Garden, Key Lab South China Agr Plant Mol Anal & Genet Im, Xingke Rd 723, Guangzhou 510650, Guangdong, Peoples R China|Univ Chinese Acad Sci, Coll Adv Agr Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China;

    Guangdong Food & Drug Vocat Coll, Longdongbei Rd 321, Guangzhou 510520, Guangdong, Peoples R China;

    Chinese Acad Sci, South China Bot Garden, Guangdong Prov Key Lab Appl Bot, Xingke Rd 723, Guangzhou 510650, Guangdong, Peoples R China|Chinese Acad Sci, South China Bot Garden, Key Lab South China Agr Plant Mol Anal & Genet Im, Xingke Rd 723, Guangzhou 510650, Guangdong, Peoples R China|Univ Chinese Acad Sci, Coll Adv Agr Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Aroma; Camellia sinensis; Chiral; 1-Phenylethanol; Short chain dehydrogenase; Tea;

    机译：芳香;山茶;手性;1-苯乙醇;短链脱氢酶;茶;

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