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Mechanism of inactivation of Bacillus subtilis spores by high pressure CO_2 at high temperature

机译：高压CO_2在高温下灭活枯草芽孢杆菌孢子的机理

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摘要

Spores of wild-type Bacillus subtilis and some isogenic mutant strains were treated by high pressure CO2 (HPCD) at high temperature (HT) (HPCD + HT) at 20 MPa and 84-86 degrees C for 0-60 min, and Centrifuged on a high density solution to obtain pelleted spores that retained CaDPA and light spores that lost CaDPA. All treated spores were analyzed for viability, and tested for germination, outgrowth, Core protein damage, mutagenesis and inner membrane (IM) properties. The results showed that (i) with HPCD + HT treated spores, most pelleted spores and all light spores were dead; ii) a significant amount of dead HPCD + HT-treated spores that retained CaDPA germinated, but outgrowth was blocked; (iii) minimal mutants were generated in survivors of HPCD + HT treatment; (iv) the GFP fluorescence decrease in HPCD + HT-treated spores with high GFP levels was slower than spore inactivation; (v) the IM of HPCD + HT-treated spores that retained CaDPA lost its ability to retain CaDPA at 85 degrees C, and almost all of these spores' outgrowth in high salt was blocked; and (vi) HPCD + HT-treated spores that retained CaDPA germinated with L-valine or AGFK were almost all stained with propidium iodide. These results indicated that HPCD + HT inactivated B. subtilis spores by damaging spores' IM, thus blocking spore outgrowth after germination.
机译：将野生型枯草芽孢杆菌的孢子和一些同基因突变体菌株在20 MPa和84-86摄氏度的高温下（HT）（HPCD + HT）用高压CO2（HPCD）处理0-60分钟，然后离心。高密度溶液以获得保留CaDPA的颗粒状孢子和失去CaDPA的轻质孢子。分析所有处理过的孢子的存活力，并测试其萌发，生长，核心蛋白损伤，诱变和内膜（IM）特性。结果表明：（i）用HPCD + HT处理的孢子，大多数颗粒状孢子和所有轻孢子均死亡。 ii）大量死去的HPCD + HT处理的孢子使CaDPA保持发芽，但阻止了长出； （iii）在HPCD + HT处理的幸存者中产生了最少的突变体； （iv）在具有高GFP水平的HPCD + HT处理的孢子中，GFP荧光的降低比灭活孢子要慢。 （v）保留CaDPA的HPCD + HT处理的孢子的IM失去了其在85摄氏度下保留CaDPA的能力，并且几乎所有这些孢子在高盐中的生长都被阻止； （vi）HPCD + HT处理的保留了CaDPA并被L-缬氨酸或AGFK萌发的孢子几乎全部被碘化丙啶染色。这些结果表明，HPCD + HT通过破坏孢子的IM灭活了枯草芽孢杆菌的孢子，从而阻止了芽后芽孢的生长。

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  • 来源
    《Food microbiology》 |2019年第9期|36-45|共10页
  • 作者

    Rao Lei; Zhao Liang; Wang Yongtao; Chen Fang; Hu Xiaosong; Setlow Peter; Liao Xiaojun;

  • 作者单位

    China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100083, Peoples R China|Natl Engn Res Ctr Fruit & Vegetable Proc, Beijing Key Lab Food Nonthermal Proc, Beijing 100083, Peoples R China|Minist Agr, Key Lab Fruit & Vegetable Proc, Beijing 100083, Peoples R China;

    China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100083, Peoples R China|Natl Engn Res Ctr Fruit & Vegetable Proc, Beijing Key Lab Food Nonthermal Proc, Beijing 100083, Peoples R China|Minist Agr, Key Lab Fruit & Vegetable Proc, Beijing 100083, Peoples R China;

    Natl Engn Res Ctr Fruit & Vegetable Proc, Beijing Key Lab Food Nonthermal Proc, Beijing 100083, Peoples R China|Minist Agr, Key Lab Fruit & Vegetable Proc, Beijing 100083, Peoples R China;

    Natl Engn Res Ctr Fruit & Vegetable Proc, Beijing Key Lab Food Nonthermal Proc, Beijing 100083, Peoples R China|Minist Agr, Key Lab Fruit & Vegetable Proc, Beijing 100083, Peoples R China;

    China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100083, Peoples R China|Natl Engn Res Ctr Fruit & Vegetable Proc, Beijing Key Lab Food Nonthermal Proc, Beijing 100083, Peoples R China|Minist Agr, Key Lab Fruit & Vegetable Proc, Beijing 100083, Peoples R China;

    UConn Hlth, Dept Mol Biol & Biophys, Farmington, CT 06030 USA;

    China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100083, Peoples R China|Natl Engn Res Ctr Fruit & Vegetable Proc, Beijing Key Lab Food Nonthermal Proc, Beijing 100083, Peoples R China|Minist Agr, Key Lab Fruit & Vegetable Proc, Beijing 100083, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Bacillus subtilis; Spores; High pressure CO2; Inactivation; Mechanism;

    机译：枯草芽孢杆菌;孢子;高压CO2;灭活;机理;

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