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Mechanism of inactivation of Bacillus subtilis spores by high pressure CO_2 at high temperature

机译：高压CO_2在高温下枯草芽孢杆菌孢子灭活机制

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摘要

Spores of wild-type Bacillus subtilis and some isogenic mutant strains were treated by high pressure CO2 (HPCD) at high temperature (HT) (HPCD + HT) at 20 MPa and 84-86 degrees C for 0-60 min, and Centrifuged on a high density solution to obtain pelleted spores that retained CaDPA and light spores that lost CaDPA. All treated spores were analyzed for viability, and tested for germination, outgrowth, Core protein damage, mutagenesis and inner membrane (IM) properties. The results showed that (i) with HPCD + HT treated spores, most pelleted spores and all light spores were dead; ii) a significant amount of dead HPCD + HT-treated spores that retained CaDPA germinated, but outgrowth was blocked; (iii) minimal mutants were generated in survivors of HPCD + HT treatment; (iv) the GFP fluorescence decrease in HPCD + HT-treated spores with high GFP levels was slower than spore inactivation; (v) the IM of HPCD + HT-treated spores that retained CaDPA lost its ability to retain CaDPA at 85 degrees C, and almost all of these spores' outgrowth in high salt was blocked; and (vi) HPCD + HT-treated spores that retained CaDPA germinated with L-valine or AGFK were almost all stained with propidium iodide. These results indicated that HPCD + HT inactivated B. subtilis spores by damaging spores' IM, thus blocking spore outgrowth after germination.
机译：通过高温（HPCD）在20MPa和84-86℃下在高温CO 2（HPCD）和84-86℃下进行0-60分钟的高压CO 2（HPCD）处理野生型枯草芽孢杆菌和一些中源性突变菌株，并离心高密度的溶液，以获得保留损伤的椎间孢子和失去CADPA的轻质孢子的颗粒孢子。分析所有经过处理的孢子的活力，并测试萌发，产高，核心损伤，诱变和内膜（IM）性质。结果表明，（i）与HPCD + HT处理的孢子，最颗粒状孢子和所有光孢子都死了; ii）大量死亡HPCD + HT治疗的孢子保留了菌斑发芽，但封闭了过多; （iii）在HPCD + HT治疗的幸存者中产生最小突变体; （iv）具有高GFP水平的HPCD + HT处理的孢子的GFP荧光降低而不是孢子灭活; （v）保留CADPA的HPCD + HT处理孢子的IM失去了85摄氏度保留了CADPA的能力，并且在高盐中几乎所有这些孢子的产卵被封锁; （VI）HPCD + HT处理的孢子，其保留与L-缬氨酸或AgFK发芽的CADPA几乎染色碘化丙锭。这些结果表明，通过损坏孢子'IM，HPCD + HT失活的B.枯草芽孢杆菌孢子，从而阻止萌发后孢子过谱。

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  • 来源
    《Food microbiology》 |2019年第9期|36-45|共10页
  • 作者

    Rao Lei; Zhao Liang; Wang Yongtao; Chen Fang; Hu Xiaosong; Setlow Peter; Liao Xiaojun;

  • 作者单位

    China Agr Univ Coll Food Sci & Nutr Engn Beijing Adv Innovat Ctr Food Nutr & Human Hlth Beijing 100083 Peoples R China|Natl Engn Res Ctr Fruit & Vegetable Proc Beijing Key Lab Food Nonthermal Proc Beijing 100083 Peoples R China|Minist Agr Key Lab Fruit & Vegetable Proc Beijing 100083 Peoples R China;

    China Agr Univ Coll Food Sci & Nutr Engn Beijing Adv Innovat Ctr Food Nutr & Human Hlth Beijing 100083 Peoples R China|Natl Engn Res Ctr Fruit & Vegetable Proc Beijing Key Lab Food Nonthermal Proc Beijing 100083 Peoples R China|Minist Agr Key Lab Fruit & Vegetable Proc Beijing 100083 Peoples R China;

    Natl Engn Res Ctr Fruit & Vegetable Proc Beijing Key Lab Food Nonthermal Proc Beijing 100083 Peoples R China|Minist Agr Key Lab Fruit & Vegetable Proc Beijing 100083 Peoples R China;

    Natl Engn Res Ctr Fruit & Vegetable Proc Beijing Key Lab Food Nonthermal Proc Beijing 100083 Peoples R China|Minist Agr Key Lab Fruit & Vegetable Proc Beijing 100083 Peoples R China;

    China Agr Univ Coll Food Sci & Nutr Engn Beijing Adv Innovat Ctr Food Nutr & Human Hlth Beijing 100083 Peoples R China|Natl Engn Res Ctr Fruit & Vegetable Proc Beijing Key Lab Food Nonthermal Proc Beijing 100083 Peoples R China|Minist Agr Key Lab Fruit & Vegetable Proc Beijing 100083 Peoples R China;

    UConn Hlth Dept Mol Biol & Biophys Farmington CT 06030 USA;

    China Agr Univ Coll Food Sci & Nutr Engn Beijing Adv Innovat Ctr Food Nutr & Human Hlth Beijing 100083 Peoples R China|Natl Engn Res Ctr Fruit & Vegetable Proc Beijing Key Lab Food Nonthermal Proc Beijing 100083 Peoples R China|Minist Agr Key Lab Fruit & Vegetable Proc Beijing 100083 Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Bacillus subtilis; Spores; High pressure CO2; Inactivation; Mechanism;

    机译：枯草芽孢杆菌;孢子;高压CO2;灭活;机制;

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