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Identification of cereulide producing Bacillus cereus by MALDI-TOF MS

机译：MALDI-TOF MS鉴定蜡状芽孢杆菌产蜡状芽孢杆菌

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摘要

The Bacillus (B.) cereus group consists of nine recognized species which are present worldwide. B. cereus play an important role in food-borne diseases by producing different toxins. Yet, only a small percentage of B. cereus strains are able to produce the heat stable cereulide, the causative agent of emetic food poisoning. To minimize the entry of emetic B. cereus into the food chain, food business operators are dependent on efficient and reliable methods enabling the differentiation between emetic and non-emetic strains.Currently, only time-consuming cell bioassays, molecular methods and tandem mass spectrometry are available for this purpose. Thus, the aim of the present study was to establish a fast and reliable method for the differentiation between emeticon-emetic strains by MALDI-TOF MS. Selected strains/isolates of the B. cereus group as well as other Bacillus spp. (total n = 121) were cultured on sheep blood agar for 48 h before analysis.Subsequently, the cultures were directly analyzed by MALDI-TOF MS without prior extraction steps. The samples were measured in the mass range of m/z 800-1800 Da. Using ClinProTools 3.0 statistical software and Flex analysis software (Bruker Daltonics GmbH, Bremen, Germany), a differentiation between emeticonemetic isolates was possible with a rate of correct identification of 99.1% by means of the evaluation of two specific biomarkers (m/z 1171 and 1187 Da).
机译：蜡状芽孢杆菌（B.illus cereus）组由全世界公认的九种物种组成。蜡状芽孢杆菌通过产生不同的毒素在食源性疾病中起重要作用。然而，蜡样芽孢杆菌中只有一小部分能够产生热稳定的蜡样内酯，后者是催吐食物中毒的病因。为了最大程度地减少蜡状芽孢杆菌进入食物链，食品经营者必须依靠有效，可靠的方法来区分催吐菌株和非催吐菌株，目前只有耗时的细胞生物测定，分子方法和串联质谱法可用于此目的。因此，本研究的目的是建立一种快速可靠的方法，通过MALDI-TOF MS区分催吐和非催吐菌株。蜡状芽孢杆菌和其他芽孢杆菌属菌种的选定菌株/分离株。在分析之前，将（总n = 121）在绵羊血琼脂上培养48 h。随后，无需事先提取步骤即可通过MALDI-TOF MS直接分析培养物。在m / z 800-1800 Da的质量范围内测量样品。使用ClinProTools 3.0统计软件和Flex分析软件（Bruker Daltonics GmbH，不来梅，德国），可以通过对两种特定的生物标记物（m / z 1171和1187 Da）。

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  • 来源
    《Food microbiology》 |2019年第9期|75-81|共7页
  • 作者

    Ulrich Sebastian; Gottschalk Christoph; Dietrich Richard; Maertlbauer Erwin; Gareis Manfred;

  • 作者单位

    Ludwig Maximilians Univ Munchen, Vet Fac, Food Safety, Schoenleutnerstr 8, D-85764 Oberschleissheim, Germany;

    Ludwig Maximilians Univ Munchen, Vet Fac, Food Safety, Schoenleutnerstr 8, D-85764 Oberschleissheim, Germany;

    Ludwig Maximilians Univ Munchen, Vet Fac, Hyg & Technol Milk, Schoenleutnerstr 8, D-85764 Oberschleissheim, Germany;

    Ludwig Maximilians Univ Munchen, Vet Fac, Hyg & Technol Milk, Schoenleutnerstr 8, D-85764 Oberschleissheim, Germany;

    Ludwig Maximilians Univ Munchen, Vet Fac, Food Safety, Schoenleutnerstr 8, D-85764 Oberschleissheim, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    MALDI-TOF MS; Bacillus cereus; Cereulide; Food intoxication;

    机译：MALDI-TOF MS;蜡状芽孢杆菌;蜡状内酯;食物中毒;

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