• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Food microbiology >Biovolume and spatial distribution of foodborne Gram-negative and Gram-positive pathogenic bacteria in mono- and dual-species biofilms
【24h】

Biovolume and spatial distribution of foodborne Gram-negative and Gram-positive pathogenic bacteria in mono- and dual-species biofilms

机译：单体和双物质生物膜中食素革兰阴性和革兰氏阳性致病细菌的生物弱和空间分布

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The objective of this study was to characterize the biofilms formed by Salmonella enterica serotype Agona, Listeria monocytogenes, methicillin-resistant Staphylococcus aweus (MRSA) and vancomycin-resistant Enterococcus faecium (VRE) after 12, 48, 72, 120 and 240 h of incubation at 10 °C. Biofilms containing a single species, together with dual-species biofilms in which S. enterica and a Gram-positive bacterium existed in combination, were formed on polystyrene and evaluated by using confocal laser scanning microscopy (CLSM). All strains were able to form biofilm. The greatest biovolume in the observation field of 14,161 μm~2 was observed for mono-species biofilms after 72 h, where biovolumes of 94,409.0 μm~3 ± 2131.0 μm~3 (S. enterica), 58,418.3 μm~3 ± 5944.9 μm~3 (I. monocytogenes), 68,020.8 μm~3 ± 5812.3 μm~3 (MRSA) and 59,280.0 μm~3 ± 4032.9 μm~3 (VRE) were obtained. In comparison with single-species biofilms, the biovolume of S. enterica was higher in the presence of MRSA or VRE after 48, 72 and 120 h. In dual-species biofilms, the bacteria showed a double-layer distribution pattern, with S. enterica in the top layer and Gram-positive bacteria in the bottom layer. This spatial disposition should be taken into account when effective strategies to eliminate biofilms are being developed.
机译：本研究的目的是表征由Salmonella肠溶型Agona，Histeria单核细胞增生，甲氧西林的葡萄球菌（MRSA）和抗病毒素抗性肠球菌（VRE）孵育后的生物膜的特征在于12,48,72,120和240小时在10°C。在聚苯乙烯上形成含有单个物种的生物膜，其中与双种生物膜组合，其中肠溶和革兰氏阳性细菌，并通过使用共聚焦激光扫描显微镜（CLSM）评价。所有菌株都能够形成生物膜。 72小时后，观察到14,161μm〜2的观察领域的最大生物剧集在72小时后，其中患者为94,409.0μm〜3±2131.0μm〜3（s肠溶），58,418.3μm〜3±5944.9μm〜3 （I.单核细胞元），获得68,020.8μm〜3±5812.3μm〜3（MRSA）和59,280.0μm〜3±4032.9μm〜3（VE）。与单一物种生物膜相比，在48,72和120h之后的MRSA或VE存在下，S.肠的生物粒子较高。在双面生物膜中，细菌显示双层分布图案，在顶层和底层的革兰氏菌中的S.肠。当正在开发出消除生物膜的有效策略时，应考虑这种空间配置。

著录项

  • 来源
    《Food microbiology》 |2021年第4期|103616.1-103616.8|共8页
  • 作者

    Cristina Rodriguez-Melcon; Alicia Alonso-Hernando; Felix Riesco-Pelaez; Camino Garcfa-Fernandez; Carlos Alonso-Calleja; Rosa Capita;

  • 作者单位

    Department of Food Hygiene and Technology Veterinary Faculty University of Leon E-24071 Leon Spain Institute of Food Science and Technology University of Leon E-24071 Leon Spain;

    Department of Food Hygiene and Technology Veterinary Faculty University of Leon E-24071 Leon Spain Facultad de Ciencias de la Salud Universidad Isabel 1 E-09003 Burgos Spain;

    Department of Electrical Engineering and Systems Engineering and Automatic Control University of Leon E-24071 Leon Spain;

    Department of Food Hygiene and Technology Veterinary Faculty University of Leon E-24071 Leon Spain Institute of Food Science and Technology University of Leon E-24071 Leon Spain;

    Department of Food Hygiene and Technology Veterinary Faculty University of Leon E-24071 Leon Spain Institute of Food Science and Technology University of Leon E-24071 Leon Spain;

    Department of Food Hygiene and Technology Veterinary Faculty University of Leon E-24071 Leon Spain Institute of Food Science and Technology University of Leon E-24071 Leon Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Salmonella enterica; Listeria monocytogenes; MRSA; VRE; Single-species biofilms; Dual-species biofilms; Biofilm architecture;

    机译：沙门氏菌肠道;李斯特菌;MRSA;vere;单物种生物膜;双物种生物膜;生物膜建筑;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号