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Reassessment of Cronobacter spp. originally isolated as Enterobacter sakazakii from infant food

机译：克氏杆菌属物种的重新评估。最初从婴儿食品中分离为阪崎肠杆菌

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摘要

Cronobacter spp. cause infant disease, several cases have been associated with powdered infant formulae (PIF). In the early 2000s, contamination of German PIF with these opportunistic pathogens was quite common. Before 2008, all isolates Cronobacter spp. had been classified as Enterobacter sakazakii, therefore little is known about species diversity within such isolates. Genetic, serologic, and biochemical traits of 80 Cronobacter isolates, originally obtained 2003-2006 within infant food surveys in Germany, were reassessed in this study. By sequencing of the fusA gene, all isolates were unambiguously assigned to two species, C. sakazakii (n = 73) and C. malonaticus (n = 7). PCR serotyping identified five C. sakazakii serotypes and two C. malonaticus serotypes, biochemical profiling yielded five biogroups. PFGE analysis also showed high heterogeneity in both species. Multilocus sequence typing of 26 selected isolates yielded 16 different sequence types (ST), including C. sakazakii ST 1 (n = 6) and the highly virulent ST 4 (n = 2). The results suggest that just two, but highly heterogeneous species were responsible for the Cronobacter contamination problem which challenged the German PIF industry in the beginning of this century. This fact may have influenced the success of efforts to identify and eliminate sources of contamination.
机译：克氏杆菌属。导致婴儿疾病的原因包括粉状婴儿配方奶粉（PIF）。在2000年代初期，德国PIF被这些机会病原体污染非常普遍。在2008年之前，所有分离株均属于Cronobacter spp。被分类为阪崎肠杆菌，因此对这种分离物中物种的多样性知之甚少。这项研究重新评估了最初在德国婴儿食品调查中于2003-2006年获得的80种克氏杆菌分离株的遗传，血清学和生化特性。通过对fusA基因进行测序，所有分离株都明确地分为两种：阪崎肠杆菌（n = 73）和丙酸梭菌（n = 7）。 PCR血清分型鉴定出五种阪崎肠杆菌血清型和两种丙二酸丙酸杆菌血清型，生化分析产生了五个生物组。 PFGE分析还显示了两个物种的高度异质性。对26个选定的分离株进行多基因座序列分型产生了16种不同的序列类型（ST），包括阪崎肠杆菌ST 1（n = 6）和高毒力ST 4（n = 2）。结果表明，只有两个但高度异质的物种造成了克罗诺杆菌污染问题，该问题在本世纪初挑战了德国的PIF工业。这个事实可能影响了识别和消除污染源的努力的成功。

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  • 来源
    《Food microbiology》 |2017年第8期|44-50|共7页
  • 作者

    Ömer Akineden; Vanessa Heinrich; Madeleine Gross; Ewald Usleber;

  • 作者单位

    Chair of Dairy Science, Institute of Veterinary Food Science, Justus-Liebig University Giessen, Ludwigstrasse 21, D-35390, Ciessen, Germany;

    Chair of Dairy Science, Institute of Veterinary Food Science, Justus-Liebig University Giessen, Ludwigstrasse 21, D-35390, Ciessen, Germany;

    Junior Professorship of Veterinary Food Diagnostics, Institute of Veterinary Food Science, Justus-Liebig University Giessen, Ludwigstrasse 21, D-35390, Ciessen, Germany;

    Chair of Dairy Science, Institute of Veterinary Food Science, Justus-Liebig University Giessen, Ludwigstrasse 21, D-35390, Ciessen, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Keyworas: Cronobacter spp; Enterobacter sakazakii; Genotyping; Biotyping; Serotyping; Powdered infant formula; Germany;

    机译：Keyworas：克氏杆菌属;阪崎肠杆菌;基因分型生物分型;血清分型;婴儿配方粉;德国;

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