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首页> 外文期刊>Food microbiology >Biocide tolerance, phenotypic and molecular response of lactic acid bacteria isolated from naturally-fermented Alorena table to different physico-chemical stresses
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Biocide tolerance, phenotypic and molecular response of lactic acid bacteria isolated from naturally-fermented Alorena table to different physico-chemical stresses

机译：从自然发酵的Alorena表中分离出的乳酸菌对不同理化胁迫的杀菌剂耐受性，表型和分子响应

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摘要

Lactic acid bacteria (LAB) isolated throughout the fermentation process of Alorena table olives were found to be resistant at least to three antibiotics (Casado Munoz et al., 2014); however, most were sensitive to the biocides tested in this study (with minimum inhibitory concentrations [MIC] below the epidemiological cut-off values). 2-15% of the isolates were found to be biocide resistant: Leuconostoc Pseudomesenteroides, which were resistant to hexachlorophene, and Lactobacillus pentosus to cetrimide and hexadecylpiridinium. We analyzed the effect of different physico-chemical stresses, including antimicrobials, on the phenotypic and genotypic responses of LAB, providing new insights on how they become resistant in a changing environment. Results indicated that similar phenotypic responses were obtained under three stress conditions: antimicrobials, chemicals and UV light. Susceptibility patterns to antibiotics changed: increasing MICs for ampicillin, chloramphenicol, ciprofloxacin, teicoplanin and tetracycline, and decreasing the MICs for clindamycin, erythromycin, streptomycin and trimethoprim in most strains. Statistically, cross resistance between different antibiotics was detected in all stress conditions. However, expression profiles of selected genes involved in stress/resistance response (rpsL, recA, uvrB and srtA) differed depending on the stress parameter, LAB species and strain, and the target gene. We conclude that, despite the uniform phenotypic response to stresses, the repertoire of induced and repressed genes differs. So, a search for a target to improve stress tolerance of LAB, especially those of importance as starter/protective cultures or probiotics, may depend on the individual screening of each strain, even though we could predict the antibiotic phenotypic response to all stresses.
机译：发现在Alorena食用橄榄的整个发酵过程中分离出的乳酸菌（LAB）至少对三种抗生素具有抗性（Casado Munoz等人，2014）；然而，大多数对本研究中测试的杀生物剂敏感（最低抑制浓度[MIC]低于流行病学临界值）。发现有2-15％的分离株具有抗生物杀灭剂的能力：对六氯酚有抗性的Leuconostoc Pseudomesenteroides和对西曲美肽和十六烷基哌啶鎓具有抗性的戊糖乳杆菌。我们分析了不同的物理化学应激（包括抗菌素）对LAB的表型和基因型反应的影响，从而提供了有关它们如何在变化的环境中变得耐药的新见解。结果表明在三种胁迫条件下获得了相似的表型反应：抗菌剂，化学药品和紫外线。抗生素的敏感性模式发生了变化：在大多数菌株中，氨苄青霉素，氯霉素，环丙沙星，替考拉宁和四环素的MIC增加，而克林霉素，红霉素，链霉素和甲氧苄啶的MIC降低。从统计学上讲，在所有压力条件下均检测到不同抗生素之间的交叉耐药性。但是，根据胁迫参数，LAB种类和菌株以及目标基因，参与胁迫/抗性应答的选定基因（rpsL，recA，uvrB和srtA）的表达谱会有所不同。我们得出的结论是，尽管对压力有统一的表型反应，但诱导和抑制基因的库却有所不同。因此，即使我们可以预测出对所有压力的抗生素表型反应，寻找能够提高LAB压力耐受性的目标，尤其是作为起子/保护性培养物或益生菌的目标，也可能取决于每种菌株的个体筛选。

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  • 来源
    《Food microbiology》 |2016年第12期|1-12|共12页
  • 作者

    Maria del Carmen Casado Munoz; Nabil Benomar; Leyre Lavilla Lerma; Charles W. Knapp; Antonio Galvez; Hikmate Abriouel;

  • 作者单位

    Area de Microbiologia, Departamento de Ciencias de la Salud. Facultad de Ciencias Experimentales, Universidad de Jaen, 23071, Jaen, Spain;

    Area de Microbiologia, Departamento de Ciencias de la Salud. Facultad de Ciencias Experimentales, Universidad de Jaen, 23071, Jaen, Spain;

    Area de Microbiologia, Departamento de Ciencias de la Salud. Facultad de Ciencias Experimentales, Universidad de Jaen, 23071, Jaen, Spain;

    Department of Civil and Environmental Engineering, University of Strathclyde, Glasgow, Scotland, United Kingdom;

    Area de Microbiologia, Departamento de Ciencias de la Salud. Facultad de Ciencias Experimentales, Universidad de Jaen, 23071, Jaen, Spain;

    Area de Microbiologia, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Edif. B3, Universidad de Jaen, Campus Las Lagunillas s, 23071, Jaen, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Lactobacillus pentosus; Leuconostoc pseudomesenteroides; Antibiotics; Biocides; Stress; Quantitative PCR; Gene expression;

    机译：戊糖乳杆菌;假性肠球菌抗生素;杀菌剂;强调;定量PCR;基因表达;

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