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首页> 外文期刊>Food microbiology >A new in vitro method to detect growth and ochratoxin A-producing ability of multiple fungal species commonly found in food commodities
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A new in vitro method to detect growth and ochratoxin A-producing ability of multiple fungal species commonly found in food commodities

机译：检测食品中常见的多种真菌物种生长和曲霉毒素A产生能力的体外新方法

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摘要

The aim of the study was to develop a new screening method to detect growth and ochratoxin A (OTA) production by multiple fungi growing in a small quantity of culture media, using microtiter plates. Eight ochratoxigenic species were included in the study. The strains were inoculated in sterile 96-well flat-bottom microtiter plates containing Yeast Extract Sucrose broth and Czapek Yeast Extract broth and incubated at 25℃. Growth was daily monitored by absorbance measurements for 4 days and extended to 7 and 10 days for Penicillium spp. The entire experiment was repeated twice on different days. On each sampling time, five of the seven replicate wells inoculated for each strain and culture media were randomly selected and the content of each well was removed, extracted and injected into the HPLC. No statistically significant differences were observed for absorbance and OTA values, neither between replicates nor between experiments. Quantifiable OTA levels were detected after 48 h of incubation in Aspergillus alliaceus, Aspergillus carbonarius and Aspergillus niger, after 72 h in Aspergillus flocculosus, Aspergillus steynii and Aspergillus westerdijkiae and after 7 days in Penicillium nordicum and Penicillium verrucosum. The method offers the necessary tools for a rapid detection of growth and OTA production avoiding the use of plate cultures and can be very useful when many fungal isolates need to be screened.
机译：该研究的目的是开发一种新的筛选方法，使用微量滴定板检测在少量培养基中生长的多种真菌的生长和曲霉毒素A（OTA）的产生。该研究包括八种产毒毒素的物种。将菌株接种在含有酵母提取物蔗糖肉汤和Czapek酵母提取物肉汤的无菌96孔平底微量滴定板中，并在25℃下孵育。每天通过吸光度测量监测生长4天，而对于青霉属物种则延长至7天和10天。整个实验在不同的日期重复两次。在每个采样时间，随机选择为每种菌株和培养基接种的七个重复孔中的五个，然后除去每个孔的内容物，提取并注入到HPLC中。无论是重复实验还是实验之间，吸光度和OTA值均无统计学差异。在小曲霉，碳曲霉和黑曲霉中培养48小时后，在絮凝曲霉，史氏曲霉和西伯利亚曲霉中培养72小时，然后在北dic青霉和疣青霉中培养7天后，检测到可量化的OTA水平。该方法提供了用于快速检测生长和OTA产生的必要工具，从而避免了使用平板培养物，并且在需要筛选许多真菌分离株时非常有用。

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  • 来源
    《Food microbiology》 |2014年第12期|243-248|共6页
  • 作者

    M.L. Abarca; M.R. Bragulat; F.J. Cabanes;

  • 作者单位

    Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autonoma de Barcelona, Bellaterra, Spain,Grup de Micologia Vetennaria, Departament de Sanitat i d Anatomia Animals, Facultat de Veterinaria, Universitat Autdnoma de Barcelona, 08193 Bellaterra, Barcelona, Spain;

    Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autonoma de Barcelona, Bellaterra, Spain;

    Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autonoma de Barcelona, Bellaterra, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Aspergillus; Growth detection; Inoculum standardization; Ochratoxin A; OTA-producing ability; Penicillium; Screening method; Spectrophotometric measurement of growth;

    机译：曲霉;生长检测;接种标本;ch曲毒素A;OTA产生能力;青霉;筛选方法;分光光度法测量生长;

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