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Grape seed extract for foodborne virus reduction on produce

机译:葡萄籽提取物可减少食品中的食源性病毒

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Grape seed extract (GSE) is reported to have antibacterial properties with few current studies on antiviral activity. Recently, we reported the effects of GSE against foodborne viral surrogates in vitro. This study evaluated the application of GSE (commercial Gravinol-S) against hepatitis A virus (HAV) and human norovirus surrogates, feline calicivirus (FCV-F9) and murine norovirus (MNV-1), on model produce. Washed and air-dried lettuce (3×3 cm~2) and jalapeno peppers (25-30 g) were inoculated with FCV-F9, MNV-1, or HAV at high (~ 7 log_(10)PFU/ml) or low (~ 5 logro PFU/ml) titers, and treated with 0.25, 0.5, 1 mg/ml GSE or water for 30 s to 5 min. Treatments were stopped/diluted with cell-culture media containing 10% heat-inactivated fetal bovine serum and evaluated using plaque assays. At high titers, FCV-F9 was reduced by 2.33, 2.58, and 2.71 logio PFU on lettuce; and 2.20,2.74, and 3.05 log_(10) PFU on peppers after 1 min using 0.25, 0.50, and 1 mg/ml GSE, respectively. Low FCV-F9 titers could not be detected after 1 min at all three GSE concentrations. Low titer MNV-1 was reduced by 0.2-0.3 log_(10) PFU on lettuce and 0.8 log_(10) PFU on peppers, without reduction of high titer. GSE at 0.25-1 mg/ml after 1 min caused 0.7-1.1 and 1-1.3 log_(10) PFU reduction for high and low HAV titers, respectively on both commodities. Instrumental color analysis showed no significant differences between treated and untreated produce. GSE shows potential for foodborne viral reduction on produce as part of hurdle technologies.
机译:据报道,葡萄籽提取物(GSE)具有抗菌特性,目前对抗病毒活性的研究很少。最近,我们报道了GSE在体外对食源性病毒替代物的影响。这项研究评估了GSE(商品Gravinol-S)对甲型肝炎病毒(HAV)和人类诺如病毒替代品,猫杯状病毒(FCV-F9)和鼠诺如病毒(MNV-1)在模型生产中的应用。用高(〜7 log_(10)PFU / ml)的FCV-F9,MNV-1或HAV接种洗净风干的生菜(3×3 cm〜2)和墨西哥胡椒(25-30 g)低(〜5 logro PFU / ml)滴度,并用0.25、0.5、1 mg / ml GSE或水处理30 s至5分钟。用含有10%热灭活胎牛血清的细胞培养基停止/稀释治疗,并使用噬斑测定法进行评估。高滴度时,生菜中的FCV-F9降低了2.33、2.58和2.71 logio PFU。 1分钟后分别使用0.25、0.50和1 mg / ml GSE在辣椒上的PFU分别为2.20、2.74和3.05 log_(10)PFU。在所有三种GSE浓度下1分钟后都未检测到低的FCV-F9滴度。低滴度MNV-1在生菜上降低了0.2-0.3 log_(10)PFU,在辣椒上降低了0.8 log_(10)PFU,而高滴度却没有降低。 1分钟后GSE浓度为0.25-1 mg / ml时,两种商品的高和低HAV效价分别降低0.7-1.1和1-1.3 log_(10)PFU降低。仪器颜色分析表明,处理过的和未处理过的产品之间没有显着差异。 GSE作为障碍技术的一部分,显示出减少农产品中食源性病毒的潜力。

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